错配修复基因hMSH_2启动子甲基化与胶质瘤的关系

Relationship between Mismatch Repair Gene hMSH_2 Promoter Methylation and Glioma

目的探讨hMSH2基因启动子区高甲基化在胶质瘤发生发展中的作用。方法应用甲基化特异性PCR(methylation specific PCR,MSP)方法检测胶质瘤组织(实验组)及脑外伤组织(对照组)中hMSH2基因启动子区甲基化状态。结果实验组82例中,42例存在启动子的甲基化;对照组20例中,有5例存在启动子甲基化。实验组与对照组甲基化水平相比,差别有统计学意义(χ2=4.449,P=0.035)。hMSH2启动子甲基化状态与不同级别的胶质瘤之间存在相关性(χ2=7.280,P=0.007)。结论胶质瘤中,hMSH2基因启动子区高甲基化,不但可作为判断胶质瘤进展的指标之一,而且还可能是导致其错配修复功能缺陷,引发微卫星不稳定现象,从而诱发肿瘤的重要因素。

Objective To discuss the influences of hMSH2 gene promoter high methylation reacts with glioma.Methods To apply methylation specific PCR method to test hMSH2 gene promoter area methylation status between glioma organization（experimental group） and brain injury organization（contrast group）.Results Among the 82 patients,42 patients had promoter methylation.Among the 20 patients in the contrast group,5 patients had promoter methylation.Methylation levels are compared between the experimental group and the contrast group,the distinction is of statistical significance（χ2=4.449,P=0.035）.hMSH2 gene promoter methylation is related to the different grades of tumor of glioma（χ2=7.280,P=0.007）.Conclusion hMSH2 gene promoter high methylation in glioma not only could be seen as one of the indicators for judging development of glioma,but also may cause defects in mismatch repairing function which may further cause unstable microsatellite as an important factor in inducing tumors.