藏红花素对K562细胞株及裸鼠移植瘤的抑制作用的研究

Inhibitory effect of crocin on K562 cells and Balb/c nude mice xenograft tumor

目的探讨藏红花素对K562细胞株及裸鼠移植瘤的作用。方法利用MTT法检测不同浓度藏红花素对K562细胞的增殖抑制影响;Annexin V-FITC标记法检测K562细胞凋亡;建立K562细胞Balb/c裸鼠皮下移植瘤模型,荷瘤小鼠随机分成4组,分别用10、50、100 mmol/L浓度的藏红花素和生理盐水对瘤体进行局部注射,每次0.1 ml,隔日1次,连续7次,观察瘤体变化。结果不同浓度藏红花素对K562细胞均有抑制作用,呈明显的剂量依赖性,抑制率分别为21.5%、55.1%、81.6%(P<0.05);浓度为0.003 2 mol/L的藏红花素诱导K562细胞凋亡作用最显著,浓度为6.4 mmol/L的藏红花素使K562细胞呈中毒反应,凋亡作用不明显;藏红花素组瘤体生长较对照组明显受到抑制,不同浓度的藏红花素抑瘤率分别为18.5%、79.2%、91.6%。结论藏红花素具有显著抑制K562细胞增殖及促凋亡的作用,能有效抑制K562细胞Balb/c裸鼠移植瘤的生长。

Objective To study the anti-tumor effect of crocin on human leukemia cell line K562 and Balb/c nude mice xenograft tumor.Methods MTT cell proliferation assay was used to evaluate the anti-tumor effect of crocin.The quantization of cells apoptosis was determined by Annexin V-FITC marking method.The model of mouse xenograft tumor was established by injecting human leukemia cancer cell line K562 into the subcutaneous tissue of nude mice.The tumor body of tumor-bearing mice was injected every other day for 7 days with 0.1 ml normal saline solution,0.01M,0.05M and 0.1M crocin,respectively.Results The rates of growth inhibition were 21.5%,55.1% and 81.6% respectively for K562 cells treated with 0.0008M,0.0016M and 0.0032M crocin for 24 hrs（P0.05）.The growth inhibition effect of crocin was dose dependent.The apoptosis of K562 cells was obvious after 24 hours treatment with 0.0032M crocin.The inhibition rates of Balb/c nude mice xenograft tumor were 18.5%,79.2%% and 91.6% respectively after 7-day treatment with 0.01M,0.05M and 0.1M crocin.Conclusion Crocin induces tumor cells apoptosis and effectively inhibits the growth of K562 cell line and Balb/c nude mice xenograft tumor in vivo.