摘要
目的观察尿胰蛋白酶抑制剂（UTI）对人肝癌细胞株SK-HEP-1迁移和侵袭等肿瘤生物学特性的影响。方法噻唑蓝（MTY）比色法检测UT1对SK—HEP-1细胞增殖的影响；Transwell法检测不同浓度UTI对细胞迁移和侵袭能力的作用；逆转录一聚合酶链反应（RT—PCR）和Western blot检测不同浓度uTI作用后，尿激酶型纤溶酶原激活物（UPA）的mRNA和蛋白表达。结果UTI组对SK-HEP-1细胞的增殖无影响（P〉0．05）；与对照组比较，不同浓度的UTI组均能抑制人肝癌细胞SK—HEP-1的体外迁移和侵袭能力，且呈剂量效应关系，差异有统计学意义（P〈0．05）；不同浓度的UTI组中SK—HEP-1细胞UPA的mRNA和蛋白表达均明显下降，且呈剂量依赖性，与对照组比较差异有统计学意义（P〈0．05）。结论uTI能抑制人肝癌细胞SK—HEP-1迁移与侵袭，其机制可能与UTI抑制SK—HEP-1细胞UPA表达有关。
Objective To investigate the effects of urinary trypsin inhibitor （UTI） on the proliferation, migration and invasion of SK-HEP-1 hepatocellular carcinoma cells in vitro. Methods Effects of UTI on SK-HEP-1 cells proliferation was evaluated by methyl thiazol tetrazolium （MTF） assay; Effects of migration and invasion by UTI on SK-HEP-1 cells was evaluated by Transwell assay ; The expression of urokinase type plasminogen activator （UPA） after UTI administration was evaluated by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting. Results UTI did not affect the proliferation of SK-HEP-1 ceils （P 〉 0. 05）. UTI suppressed the migration and invasion of SK-HEP-1 cells dose-dependantly（P 〈 0. 05）. UTI suppressed UPA expression of SK-HEP-1 on mRNA and protein level dose-dependantly（P 〈 0. 05 ）, as compared with the control group. Conclusion UTI can suppress the migration and invasion of SK-HEP-1 hepatocellular cells. Decreasing the expression of UPA by UTI may be relevant to the mechanism of the migration and invasion suppression on SK-HEP-1 cells by UTI.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第11期1881-1883,共3页
Chinese Journal of Experimental Surgery
基金
天普研究基金资助项目（01200907）
