大鼠同种骨髓间充质细胞门静脉输注对肝大部分切除肝衰模型术后肝功能、生存率的影响

Research on the effect of allogeneic MSC transplantation through portal vein in rat partial hepatectomy model

目的观察大鼠同种骨髓间充质细胞（BMSCs）经门静脉肝内移植对80％肝切除肝衰竭模型术后肝功能、生存率的影响。方法通过80％肝切除建立大鼠边缘肝衰竭模型，经门静脉输注绿荧光蛋白（GFP）标记的第5代（P5）大鼠同种MSC，观察BMSCs肝内移植对肝大部分切除边缘肝衰竭模型术后生存率、肝功能的影响。结果术后7d内的存活率：MSC组72．2％（13／18）、对照组44．4％（8／18）。MSC组的存活率明显高于对照组（72．2％比44．4％，P〈0．05；MSC组大鼠术后肝功能恢复情况明显优于对照组（P〈0．05）；MSC组肝组织中可观察到移植的细胞。结论经门静脉的BMSCs移植可促进80％肝切除边缘肝衰竭大鼠的肝功能恢复和提高生存率。

Objective To investigate the effect of liver function and survival rate on allogeneic bone mesenchymal stem cells （BMSCs） transplantation through portal vein in the rat 80% hepatectomy model. Methods Build up the edge of limit hepatectomy rat model, transplant the 5th passage （P5） allogeneic rat BMSCs marked with green fluorescent protein （ GFP）, observe the effect of liver function and survival rate after allogeneic BMSCs transplantation through portal vein in the rat hepatectomy model. Resuits Survival rate after 7 days ： MSC Group 72. 2% （ 13/18）, control Group 44.4% （8/18）, The survival rate of MSC Group was significantly higher than control Group （72. 2% vs. 44. 4%, P 〈 O. 05 ） ; the liver function recovery after surgery of MSC Group is better than control Group （ P 〈 0. 05 ） ; transplanted BMSCs could be deteced in recipient liver of MSC Group. Conclusion BMSCs transplanted though portal vein can promote liver function recovery and improve survival rates in rat 80% hepatectomy model.