5-氟尿嘧啶磁性白蛋白微球联合恒定外磁场对人胰腺癌细胞生长的抑制作用

Cytotoxic effects of 5-fiuorouracil magnetic albumin microspheres combined with external magnetic fields on human pancreatic carcinoma cells

目的探讨5-氟尿嘧啶磁性白蛋白微球（5-Fu—MAMS）联合恒定外磁场体外对人胰腺癌PC-3细胞生长的抑制作用及其机制。方法将体外培养人胰腺癌PC-3细胞分为5-Fu—MAMS联合磁场组；5-Fu-MAMS组；游离5-Fu组，倒置显微镜下观察肿瘤细胞的生长状态，噻唑蓝（MTT）比色分析法检测各组细胞的抑制率。结果5-Fu浓度为20．0mg／L时，5-Fu—MAMS组与游离5-Fu组抑制率分别为57．7％和59．6％，IR〉50％，药物敏感，差异无统计学意义（P〉0．05）；5-Fu浓度为10．0mg／L时，5-Fu-MAMS联合磁场能明显提高化疗药物的抑瘤效果，IR为60％，IR与其他两组比较差异有统计学意义（P〈0．05）。结论5-Fu—MAMS和游离5-Fu有相似的药理作用；联合恒定外磁场能显著增强5-Fu—MAMS的抑瘤效应。

Objective To investigate the growth inhibiting action of 5-fluorouracil magnetic albu- min microspheres （5-Fu-MAMS） combined with external magnetic fields on human pancreatic carcinoma cells in vitro, and the mechanism. Methods The cultured PC-3 cells were divided into three groups： the group of 5-Fu-MAMS combined with external magnetic fields ; the group of 5-Fu-MAMS without magnetic fields, and 5-Fu group. The growth of PC-3 cells were observed under an inverted microscopy, and the inhibitory rate （IR） was assayed by methyl thiazol tetrazolium （MTT） assay. Results The inhibitory rate of 5-Fu-MAMS group and 5-Fu group was 57.7% and 59. 6% respectively （P 〉 0. 05）. The inhibitory rate of 5-Fu-MAMS combined with external fields was significantly increased to 60% （P 〈 0. 05 ）. Conclusion There were same pharmacological action between 5-Fu-MAMS and 5-Fu. The growth inhibiting action was significantly increased after 5-Fu-MAMS combined with external magnetic fields.