神经干细胞-多肽自组装凝胶复合体移植治疗脊髓损伤

Effects of co-transplatation of neural stem cells and the self-assembly peptide nanofiber hydrogels on the repair of transversal spinal cord injury in rats

目的观察神经干细胞（NSCs）复合多肽自组装凝胶移植对大鼠脊髓损伤（SCI）后功能修复的影响。方法36只SD大鼠造模后1周随机分为3组，分别为DMEM／F12对照组（n=12）、NSCs移植组（n=12）和NSCs-凝胶移植组（n=12）。通过不同时间点BBB评分、病理组织学、免疫荧光技术评价脊髓损伤的修复。结果移植后2周开始3组大鼠各时间点评分差异有统计学意义（P〈0．01），且组间差异均有统计学意义（P〈0．01）；移植后6周，病理切片示C组大量再生的神经纤维桥接脊髓断端，胶质瘢痕不明显；免疫荧光染色示C组5-溴脱氧尿嘧啶核苷（5-BrdU）／NF-200双标阳性细胞比例（24．83±1．47）％明显多于B组（6．83±1．47）％（P〈0．01），但B组BrdU／GFAP双标阳性细胞比例（42．17±2．71）％明显多于C组（34．33±4．63）％（P〈0．01）。结论自组装多肽凝胶能提高神经干细胞向神经元分化的比例，复合移植能更有效地促进脊髓功能恢复。

Objective To study the effects of co-transplatation of neural stem cells （NSCs） and the self-assembly peptide nanofiber hydrogels on the repair of transversal spinal cord injury in rats. Methods Thirty-six SD rats were randomly derided into groups A, B and C, which included 12 rats in each group after the animal models of spinal cord completely transsected had been established for one week. DMEM/F12 fluid, NSCs and composition of cells and hydrogels were transplanted into the caulal zone of the transected spinal cord in three groups repcctively. The function repair was evaluated by Bundle branch block （BBB） score, pathology and imunnoflurorescence technique. Results The BBB scores in the three groups had significant difference at differernt time points （P 〈 0. 01 ）, and the BBB scores were significantly different among the three groups （ P 〈 0. 01 ） after having been transplanted for two weeks. A great amount of regeneration host neuropils bridging at the two sides of the injured area and lesser glial scar were observed in group C after having been transplanted for six weeks. The percentage of BrdU/NF-200 double positive cells in group C （24. 83 ± 1.47 ） % was higher than that in group B （6. 83 ± 1.47 ） % （ P 〈 0.01 ）. However, the percentage of BrdU/GFAP double positive cells in group B （42. 17± 2. 71 ）% was higher than that in group C （34. 33 ±4. 63）% （P 〈0. 01 ）. Conclusion The self-assembly peptide nanofiber hydrogels could improve the differentiation of NSCs into neurons. Co-transplantation of NSCs with self-assembly of IKVAV peptide nanofiber hydrogels showed more effective results.