益神启窍方对实验昏迷大鼠昏迷状态的影响

Effect of Yishen Qiqiao Prescription on Coma State of Experimental Rats

目的:观察益神启窍方对弥漫性轴突损伤合并局灶性脑挫伤大鼠昏迷状态的影响及可能机制。方法:采用"弥漫性轴突损伤合并局灶性脑挫伤动物模型"造模,取雄性SD大鼠36只,随机分为4组,正常组7只、假手术组7只、对照组12只、中药组12只,造模前1周中药组每天灌胃益神启窍方中药配方颗粒,模型组只灌胃等剂量的生理盐水,电镜观察脑组织损伤程度,和观察从麻醉到角膜反射、从麻醉到四肢爬行时间、呼吸恢复时间及神经功能障碍评分(mnss)。结果:假手术组与正常组"麻-爬"时间无显著差异(P<0.05);中药组"麻-角"时间与假手术组有显著差异(P<0.05),与对照组有显著性差异(P<0.05);中药组"麻-爬"时间与假手术组有显著差异(P<0.05),与对照组有显著性差异(P<0.05),中药组与对照组呼吸恢复时间有显著性差异(P<0.05),中药组与对照组损伤清醒后神经功能障碍评分有显著性差异(P<0.05),电镜对照组见血管外周、轴突水肿,神经纤维水肿、溶解,轴突内线粒体、胶质细胞水肿,中药组多数神经元、血管、神经纤维正常,仅见部分轴突内线粒体水肿,树突有灶性水肿,胶质细胞、血管外周水肿。结论:中药益神启窍方能缩短实验大鼠的昏迷时间、自主呼吸恢复时间,改善神经功能障碍评分和减轻脑组织损伤程度,有显著的促醒作用和脑保护作用,这可能与其具有兴奋中枢神经、改善血供、降低氧耗有关。

Objective：To observe the effect and mechanism of Yishen Qiqiao prescription on the coma state of experimental rats due to diffuse axonal injury combined with focal brain contusion.Methods：The model was built based on the animal model of diffuse axonal injury combined with focal brain contusion.36 male SD rats were randomly divided into 4 groups： normal control group（n=7）,sham-operated group（n=7）,control group（n=12）,TCD group（n=12）.TCD group were supplied with Yishen Qiqiao granule by intragastric administration every day for one week before model-building,while the control group were only supplied with isodose saline.The brain injury was observed by electron microscope.The time from anesthesia to cornea reflection,crawl,and breath recovery was recorded and the neural function disorder was evaluated.Results：There was no significant difference in the time from anesthesia to crawl between the sham-operated group and normal control group（P〈0.05）.But significant difference was observed in the time from anesthesia to cornea reflection between TCD group and sham-operated group（P〈0.05）as well as TCD group and control group（P〈0.05）.Significant difference was also observed in the time from anesthesia to crawl between TCD group and sham-operated group（P〈0.05）as well as TCD group and control group（P〈0.05）.There was significant difference in the time of breath recovery and neural function disorder evaluation between TCD group and control group（P〈0.05）.Swollen was observed in peripheral vessel,axons,nerve fibers,mitochondria and gliocytes in the axons under electron microscope in control group.The most neurons,vessels and nerve fibers were normal in TCD group,swollen was observed in some mitochondria of the axons,gliocytes,and peripheral vessel,focal swollen was observed in some dendrites.Conclusions：Yishen Qiqiao prescription has a significant role in wake-promoting and brain-protecting by shortening the time of coma and breath recovery of rats,improving the neural function disorder evaluation and alleviating the degree of brain injury,which may be related with its function of exiting central nerve,promoting blood supply and reducing oxygen consumption.