五加减正气散化裁对溃疡性结肠炎大鼠血中肿瘤坏死因子-α水平的影响

Study on Zhengqi Powder's Influence on TNF-α of Experimental Ulcerative Colitis Rats

目的:讨论五加减正气散化裁对溃疡结肠炎(UC)大鼠血中的肿瘤坏死因子-α(TNF-α)的影响。方法:实验动物分成4组分别为空白组、模型组、西药组和中药组,采用乙酸诱导法造模,观察大鼠结肠黏膜病理变化,用ELISA法检测大鼠血中TNF-α的水平。结果:空白组大鼠血中TNF-α和结肠黏膜无变化、模型组的大鼠血中TNF-α和结肠黏膜变化明显、西药组与中药组大鼠血中TNF-α和结肠黏膜无明显变化。西药组、中药组与空白组间差异无统计学意义;模型组与空白组比较,大鼠血清中TNF-α显著升高,结肠黏膜充血,水肿,糜烂并有溃疡形成。两者之间差异有统计学意义(P<0.05);模型组与西药组、中药组间大鼠血清中TNF-α显著升高,结肠黏膜充血,水肿,糜烂并有溃疡形成,差异有统计学意义(P<0.05)。结论:五加减正气散化裁能干预实验性UC大鼠血中的TNF-α的活性,对UC大鼠结肠溃疡面有修复和保护作用。

Objective：To discuss modified Zhengqi Powder influence on ulcer colitis（UC）rats in the blood tumor necrosis factor（TNF-α）.Method：Experimental animals were divided into four groups,molding by acetic acid inducement method and with ELISA method to detect rats and serum colonic mucosal tissue TNF-α level.Results：The western medicine group,the Chinese traditional medicine group and blank group have statistically significant differences among groups（P〉0.05）;the difference between the blank group and model group is statistically significant（P〈0.05）;model group and western medicine group,Chinese traditional medicine group have statistically significant difference（P〈0.05）.Conclusion：Zhengqi Powders can intervene UC rats in the blood of TNF-α inhibitory action.It can repair and protect UC rats colon ulcer surface.