摘要
A novel electrochemiluminescence (ECL) sensing approach was developed for glucose detection based on crosslinking Ru(bpy)3Cl2-doped silica nanoparticles (RuSiNPs) with glucose dehydrogenase on a glassy carbon electrode (GCE). Glutaral- dehyde and aminopropyltrimethoxysilane were used as linking agents, and chitosan was used to immobilize the composites onto the GCE surface. The ECL sensor presented good characteristics in terms of stability and reproducibility. Under opti- mized conditions, the linear response of ECL intensity to glucose concentration was valid in the range of 0.2 to 20 mmol/L (R2 = 0.9962). The application results indicated that the proposed approach is with great potential in the determination of glucose.
A novel electrochemiluminescence （ECL） sensing approach was developed for glucose detection based on crosslinking Ru（bpy）3Cl2-doped silica nanoparticles （RuSiNPs） with glucose dehydrogenase on a glassy carbon electrode （GCE）. Glutaraldehyde and aminopropyltrimethoxysilane were used as linking agents, and chitosan was used to immobilize the composites onto the GCE surface. The ECL sensor presented good characteristics in terms of stability and reproducibility. Under opti- mized conditions, the linear response of ECL intensity to glucose concentration was valid in the range of 0.2 to 20 mmol/L （R2 = 0.9962）. The application results indicated that the proposed approach is with great potential in the determination of glucose.
基金
financially supported by the Science and Technology Project of Hebei Province
