家兔超数排卵与早期胚胎发育研究

Superovulation and Development of Early-Stage Embryo in Rabbits

为了提高家兔繁殖力,使用不同剂量的FSH对家兔进行了超数排卵研究,结果表明:采用高剂量FSH处理母兔的平均排卵点数与平均卵泡发育数显著(P<0.05)高于低剂量FSH组,两种处理方法的平均卵泡囊肿数与平均卵泡数则差异不显著(P>0.05)。家兔早期胚胎发育观察结果表明:母兔交配后24h采胚所获取的胚胎大多处于1细胞期,交配后30h胚胎多处于2细胞期,交配后35h胚胎多处于4细胞期。采用52IU FSH进行超数排卵处理,对家兔的胚胎发育无影响。

The method of superovulation in rabbits was studied by using different dosages of FSH in order to improve their reproductive ability. The results showed that the average number of the ovulation points and the developed follicles on the ovaries of the does used the high dosage of FSH was obviously higher （P〈0.05） than those of the low dosage of FSH, while theaverage number of the follicles and the cystic follicles of the does used two methods was not significantly different （P〉0.05）. The observating results of early-stage embryos＇ development also indicated that most of the collected embryos were in 1-cell stage during 24 hours after the does were accepted mating, and in 2-cell stage during 30 hours after mating and in 4-cell stage during 35 hours after mating. The dosage of 52IU FSH didn＇t influence the embryos＇ development of the rabbits when it was applied to conduct their superovulation treatment.