祛风止动精简方对抽动障碍大鼠行为的影响

Effect of Qufeng Zhidong Simplified Recipe on the Behavior of the Tic Disorder Rats

目的探讨祛风止动精简方对抽动障碍(tic disorder,TD)大鼠行为的影响。方法将50只雄性SD大鼠随机分为正常组、模型组、祛风止动1号方组、祛风止动2号方组和祛风止动精简方组,每组10只。模型组和祛风止动方各组给予阿扑吗啡(APO,2mg/kg)腹腔注射复制TD模型,正常组给予等量生理盐水腹腔注射,均每天1次,连续7周;第4周时模型组及正常组加用等量生理盐水灌胃,祛风止动方各组加用相应中药灌胃,均每天1次,连续4周。中药干预第2、4周时行刻板行为实验及开野实验,并分析实验结果。结果中药干预2、4周时,与模型组比较,祛风止动方各组刻板行为评分均降低,穿越格子数、直立次数及粪便粒数减少,理毛次数增多;祛风止动方各组间上述指标比较,差异均无统计学意义(P>0.05)。结论祛风止动精简方可显著减少APO诱导的TD大鼠刻板行为评分,改善其自发活动能力,增强其适应力。

Objective To study the effects Qufeng Zhidong Simplified Recipe（QZSR） on the behavior of the tic disorder（TD） rats.Methods Fifty male SD rats were randomly divided to the normal group,the model group,the QZSR-1 group,the QZSR-2 group,and the QZSR group,10 in each group.Two mg/kg apomorphine（APO） was intraperitoneally injected to rats in the model group,the QZSR-1 group,the QZSR-2 group,and the QZSR group,while equal volume of normal saline was intraperitoneally injected to rats in the normal group,both once daily for 7 successive weeks.At the 4th week equal volume of normal saline was intraperitoneally injected to rats in the model group and the normal group,while corresponding medicinal liquid was intraperitoneally injected to those in the rest groups,both once daily for 7 successive weeks.At the 2nd and 4th week of intervention,rats＇ improvement degrees of stereotyped behavior and the open-field test were monitored,and their experimental results were analyzed.Results At the 2nd and 4th week of intervention,when compared with those of the model group,the score of stereotyped behavior decreased,the numbers of passing-panel,straightening,and dejecta pill were reduced,and the number of grooming increased in the QZSR-1 group,the QZSR-2 group,and the QZSR group.But there was no difference among the three groups（P0.05）.Conclusion QZSR could significantly reduce APO induced stereotyped behavior scores of TD rats,improve their locomotor activities,and reinforce their adaptive faculty.