摘要
目的观察丹酚酸B(salvianolic acid B,SAB)对人胚肺成纤维细胞MRC-5增殖及分泌Ⅰ型前胶原、内源性转化生长因子β1(transforming growth factor-β1,TGF-β1)的影响。方法将MRC-5细胞随机分为4组,对照组未加入TGF-β1或SAB;TGF-β1组加入10ng/mLTGF-β1;SAB1组加入10ng/mLTGF-β1和1μmol/LSAB;SAB2组加入10ng/mLTGF-β1和10μmol/LSAB。采用MTT法观察各组细胞增殖能力;放射免疫法测定各组细胞Ⅰ型前胶原蛋白含量;ELISA法检测各组细胞内源性TGF-β1水平。结果与对照组比较,TGF-β1组MTT吸光度、Ⅰ型前胶原蛋白含量及内源性TGF-β1水平均明显增加,差异有统计学意义(P<0.05)。与TGF-β1组比较,SAB1组及SAB2组细胞吸光度明显减少,Ⅰ型前胶原蛋白含量和内源性TGF-β1水平降低,且SAB2组效果优于SAB1组,差异均有统计学意义(P<0.05)。结论 SAB可以抑制TGF-β1促MRC-5细胞增殖的作用,减弱分泌胶原和内源性TGF-β1的作用,具有阻止或延缓肺纤维化进行性发展的潜能。
Objective To investigate the effect of salvianolic acid B(SAB) on the proliferation of human embryonic lung fibroblast MRC-5,and the secretion of precollagenⅠ and endogenous transforming growth factor-β1(TGF-β1).Methods The MRC-5 cells were randomly divided into four groups as follows:the control group:cells cultured with DMEM but with no TGF-β1 or SAB;the TGF-β1 group:cell cultured with 10 ng/mL TGF-β1;the SAB1 group:cell cultured with medium with 10 ng/mL TGF-β1 and 1 μmol/L SAB;the SAB2 group:cell cultured with medium with 10 ng/mL TGF-β1 and 10 μmol/L SAB.The proliferation of cells was assayed by MTT incorporation.The concentration of amino-terminal propeptide of type Ⅰ procollagen(PINP),a marker of collagen synthesis,was measured by radioimmunoassay.The endogenous TGF-β1 levels were measured using ELISA.Results The optical density,precollagenⅠ contents,and endogenous TGF-β1 levels significantly increased when compared with those of the control group(P0.05).Compared with the TGF-β1 group,the optical density was obviously lowered,the precollagenⅠ contents and endogenous TGF-β1 levels significantly decreased in the SAB1 group and the SAB2 group,and better in the SAB2 group,showing statistical difference(P0.05).Conclusions SAB could inhibit the proliferation of MRC-5 cells induced by TGF-β1,and attenuate the roles of secreting collagen and endogenous TGF-β1.It had the potential of postponing or delaying the progressive developing of pulmonary fibrosis.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2011年第10期1401-1404,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省卫生厅资助项目(No.A2008537)
广州市中医药科研项目立项资助项目(2009)
关键词
丹酚酸B
肺成纤维细胞
增殖
转化生长因子Β1
胶原
salvianolic acid B
pulmonary fibroblast
proliferation
transforming growth factor-β1
collagen