摘要
[目的]筛选出适宜玉米成熟胚组织培养的基因型和基本培养基。[方法]以9个基因型的玉米成熟胚为外植体,研究基因型与基本培养基对成熟胚愈伤组织诱导和继代的影响。[结果]试验中表现较好的基因型依次是853-35、853-209、丹34和81162;对于玉米成熟胚愈伤组织的诱导培养,MS培养基比N6培养基更适宜,而对于玉米成熟胚愈伤组织的继代培养,N6的培养基比MS培养基更适宜。[结论]进一步完善了以玉米成熟胚为外植体的组织培养体系。
[Objective] This study was to screen out suitable genotypes and basic medium for the culture of maize mature embryos.[Method] Using mature embryos of nine maize genotypes as explants,the effects of genotypes and basic medium on callus induction and subculture were investigated.[Result] The genotypes performed better in callus induction and subculture were found in turn 853-35,853-209,Dan 34 and 81162.MS medium is better than N6 medium in the callus induction from maize embryos,while N6 medium is more suitable for callus subculture.[Conclusion] Our study further improved the tissue culture system in maize with mature embryos as explants.
出处
《安徽农业科学》
CAS
北大核心
2011年第29期17767-17768,17780,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目"玉米水分
养分资源高效利用根构型的调控机理(31070224)"
国家自然科学基金项目"玉米氮磷高效吸收的根系育种策略及根系发育模型建立(30970219)"
吉林省科技厅项目"超高产
强抗逆及氮磷高效的玉米种质创新(20080203)"
关键词
玉米
成熟胚
基因型
基本培养基
愈伤组织
Maize
Mature embryos
Genotype
Basic culture medium
Callus induction
