猕猴桃根提取物体外抗氧化活性研究

Study on Antioxidant Activity of Kiwi Root Extract in Vitro

[目的]探讨猕猴桃根提取物的体外抗氧化作用。[方法]采用DPPH自由基、超氧阴离子、羟基自由基、过氧化氢和还原力反应体系,测定猕猴桃根提取物的体外抗氧化作用,并用维生素C进行对照试验。[结果]试验条件下,ERHM对DPPH自由基、超氧阴离子(O2-.)、羟基自由基(.OH)、过氧化氢(H2O2)等均有较强的清除或抑制作用,且显示较好的量效关系,同时具有一定的还原力。其消除DPPH自由基的EC50为8.03μg/ml,清除超氧阴离子(O 2-.)的EC50为1.28 mg/ml,抑制羟基自由基(.OH)能力可达69.4%,浓度为100μg/ml时,对过氧化氢(H2O2)的清除率为42%。[结论]猕猴桃根提取物具有较强的还原力,能有效清除DPPH和超氧阴离子自由基,并抑制羟基自由基的产生。所以,ERHM有效成分具有较为显著的抗氧化作用。

[Objective] The aim is to explore antioxidation of kiwi fruit root extract in vitro.[Method] Using DPPH free radicals,superoxide,hydroxyl radicals,hydrogen peroxide and reduction force reaction system to determine antioxidation of kiwi fruit root extract in vitro and comparing with vitamin C experiments.[Result] Under experimental conditions,the scavenging or inhibition of ERHM on DPPH free radicals,superoxide anion（O-2·）,hydroxyl radical（·OH） and H2O2 are stronger,and show good dose-effect relationship,also has some reducing power.The EC50 for the elimination of DPPH radical is 8.03 μg/ml and for superoxide anion（O-2·） is 1.28 mg/ml,inhibition of hydroxyl radical（·OH） capacity is up to 69.4%,when the concentration is 100 μg/ml,the clearance rate of hydrogen peroxide（H2O2） is 42%.[Conclusion] Kiwi root extract has strong reducing power,can effectively remove the DPPH and superoxide anion radicals,and inhibit the production of hydroxyl radicals.Therefore,ERHM active ingredient has the more significant antioxidant activity.