百脉根谷胱甘肽硫转移酶基因的载体构建与检测

The Carrier Construction on Glutathione Sulfur of Lotus corniculatus for Shifting Enzyme Gene and Its Testing

[目的]探讨百脉根谷胱甘肽硫转移酶基因(LcGST)的作用。[方法]通过DNA重组技术将LcGST与中间表达载体pGN连接。[结果]成功构建了植物表达载体pGN-LcGST。[结论]pGN-LcGST载体的获得为进一步研究其在异源植物中的功能奠定了基础。

[Objective] The study aimed to discuss the action of glutathione sulfur of Lotus corniculatu for shifting the enzyme gene LcGST.[Method] The LcGST was connected to the middle expression vector pGN through the DNA recombination technology.[Result] The plant expression vector pGN-LcGST was constructed successfully.[Conclusion] The acquisition of the pGN-LcGST vector laid a foundation for further study on its function in the plant with different source.