籽瓜抗氧化及抗肿瘤活性研究

Studies on Antioxidant and Anticancer Activities of Seeding Watermelon

[目的]探讨我国西部特有的籽瓜皮和瓤的抗氧化及抗肿瘤活性。[方法]利用不同溶剂对籽瓜皮和瓤分别进行分离提取,测定了不同提取组分的抗氧化与抗肿瘤活性,以及活性物质含量。通过对各组分DPPH.、ABTS.+、羟自由基(.OH)的清除能力测定来确定籽瓜皮和瓤各组分的抗氧化活性。以MTT法确定籽瓜皮和瓤各组分的抗肿瘤活性。[结果]籽瓜皮和瓤各相粗提物对DPPH.、ABTS.+、.OH均具有较强的清除作用,显示出一定的抗氧化活性和抗肿瘤活性。瓜皮和瓜瓤各相的抗氧化活性与总多酚、总黄酮含量呈正相关。籽瓜皮的抗氧化活性相对高于瓜瓤部分,而瓜瓤的抗肿瘤活性则要高于瓜皮。[结论]籽瓜皮和籽瓜瓤具有一定的抗氧化和抗肿瘤活性,可以作为一种良好的天然抗氧化剂及抗肿瘤活性物质来源。

[Objective] The study aimed to discuss the antioxidant and anticancer activities in the rind and flesh of seeding watermelon which specially grown in the northwest of China.[Method] The rind and flesh of seeding watermelon were separate and exacted by different solvents,resp.The antioxidant and anticancer activities,as well as the active substances contents in the different extracting components were determined.The antioxidant activity was confirmed through measuring the scavenging capacity of each component in the rind and flesh of seeding watermelon to DPPH·,ABTS· ＋ and OH· The anticancer activity in each component in the rind and flesh was determined by the assay of MTT.[Result] The extracts in both the rind and flesh of seeding watermelon had stronger scavenging capacity,showing some activities of antioxidant and antitumor.The antioxidant activity in the each extract from the rind and flesh was positively correlated with the content of total polyphenol and total flavonoid contents.The antioxidant activity in the rind was higher than that in flesh,while the antitumor activity in the flesh was higher than that in the rind.[Conclusion] The the rind and flesh of seeding watermelon had some antioxidant and anticancer activities,which could taken as a kind of good natural antioxidant and the antitumor activity material source.