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口腔鳞癌淋巴结转移与丝裂原活化蛋白激酶激酶4基因表达的关系及其临床意义 被引量:1

The relationship between expression of mitogen-activated protein kinase kinase 4 and metastasis in oral squamous cell carcinoma
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摘要 目的研究丝裂原活化蛋白激酶激酶4(MKK4)在口腔鳞癌(OSCC)中的表达及其与浸润转移的关系。方法应用免疫组织化学技术及逆转录-聚合酶链反应(RT-PCR)检测口腔鳞癌原发灶及淋巴结转移灶中MKK4蛋白和mRNA的表达。结果 75例石蜡包埋口腔鳞癌组织中,MKK4蛋白的表达在48例转移组高于27例无淋巴结转移组(P<0.05);在48例淋巴结转移灶MKK4蛋白的表达水平高于其原发灶(P<0.05)。MKK4蛋白表达与OSCC原发部位、肿瘤大小、组织学分级之间无统计学差异(P>0.05)。38例新鲜口腔鳞癌组织中,MKK4 mRNA的表达量在16例转移组高于22例无淋巴结转移组(P<0.01);在6例淋巴结转移灶高于16例转移组原发灶(P<0.05)。结论 MKK4蛋白及其mRNA的表达上调与口腔鳞癌转移相关;MKK4蛋白及其mRNA可能在口腔鳞癌的浸润转移过程中发挥了促进的作用。 Objective To explore the correlation between expression of mitogen-activated protein kinase kinase 4(MKK4) and metastasis of oral squamous cell carcinoma(OSCC).Methods Expression levels of MKK4 mRNA and protein were examined in surgically resected oral squamous cell carcinoma specimens and corresponding lymph nodes by semi-quantitative reverse Transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry.Results The expression of MKK4 in 48 cases of metastasis of lymph node group was significantly higher than 27 cases of without metastasis of lymph node group in 75 cases of paraffin-embedded OSCC samples(P0.05).The expression of MKK4 in 48 cases of metastatic lymph node lesions was higher than 48 cases of primary site of OSCC(P0.05).There was no correlation between the expression of MKK4 and primary site,size of tumor,histological grade(P0.05).The expression of MKK4 mRNA in 16 cases of metastasis of lymph node group was significantly higher than 22 cases of without metastasis of lymph node group in 38 cases of fresh OSCC samples(P0.01).The expression of MKK4 in 6 cases of matched metastatic lymph node lesions was higher than 16 cases of primary tumour(P0.05).Conclusion The upregulation of MKK4 protein and mRNA may be related with the invasion and metastasis of OSCC.MKK4 maybe played a promoting role in the progression and metastasis of OSCC.
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 2011年第5期555-558,共4页 West China Journal of Stomatology
基金 广西自然科学基金资助项目(桂科自0832154)
关键词 丝裂原活化蛋白激酶激酶4 口腔鳞癌 转移 mitogen-activated protein kinase kinase 4 oral squamous cell carcinoma metastasis
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参考文献10

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