摘要
目的:通过研究白细胞介素-10(IL-10)在反复自然流产和正常流产人绒毛组织和血清中的表达比较来探讨IL-10与不明原因反复自然流产的相关性及其临床意义。方法:采用免疫组织化学技术和逆转录-聚合酶链反应技术检测35例反复自然流产者(实验组)和35例正常流产者(对照组)人绒毛组织中IL-10的细胞学定位以及在mRNA水平的表达;ELISA法定量分析IL-10在两组血清中的表达。结果:免疫组织化学检测结果显示两组组织中均有IL-10阳性表达,实验组IL-10在人绒毛组织中的阳性表达率低于对照组(P<0.05)。RT-PCR结果显示实验组和对照组均有IL-10表达,对比显示实验组IL-10表达低于对照组。ELISA测定结果显示IL-10在实验组的表达低于对照组。结论:IL-10的表达在实验组早孕绒毛组织细胞学定位及mRNA水平检测均较对照组降低,在血清中的表达量也是实验组低于对照组,提示自然流产妊娠妇女存在IL-10细胞因子表达异常,IL-10可能在维持正常妊娠过程中起到重要作用。
AIM: To investigate the correlation between interleukin-10(IL-10) and recurrent spontaneous abortion as well as the significance of them through researching the comparison of histological localization of IL-10, expression comparison of IL-10 mRNA in human chorionic villi of pregnant women with recurrent spontaneous abortion and normal abortion. METHODS: To detect expression comparison of IL-10 mRNA and histological localization of IL-10 in human chorionic villi of 35 pregnant women with recurrent spontaneous abortion(experimental group) and 35 normal abortion women(control group) by reverse transcription-polymerase chain reaction and immunohistochemistry. To analysis expression of IL-10 in serum of the two groups by ELISA. RESULTS: The detection of immunohistochemistry showed that there are expressions of IL-10 both in the two groups, the expressions of IL-10 in the experimental group were lower than the control group(P〈0.05).The results of the RT-PCR showed that the expressions of IL-10 of experimental group were lower than the control group(P〈0.05). ELISA analysis showed that the expressions of IL-10 of the experimental group were lower than the control group(P〈0.05). CONCLUSION: The results of detection of immunohistochemistry and the expression of mRNA of IL-10 in human chorionic villi as well as its expressions in serum of experimental group were lower than the control group. IL-10 would play an important role in maintaining normal pregnancy.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第10期1065-1067,1071,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
西安医学院院校基金(2010FC23)
关键词
白细胞介素-10
反复自然流产
人绒毛组织
正常人工流产
正常妊娠维持
Interleukin-10(IL-10)
recurrent spontaneous abortion
human chorionic villi
normal abortion
maintaining normal pregnancy