骨髓和脂肪来源间充质干细胞的免疫调节作用

Immunomodulatory abilities of bone marrow-derived and adipose-derived mesenchymal stem cells

背景:脂肪来源的间充质干细胞是否具有和骨髓来源间充质干细胞类似的免疫调节作用?目的:观察骨髓来源和脂肪来源间充质干细胞的免疫学特征。方法:分离骨髓和脂肪来源的间充质干细胞,分别检测它们对T细胞周期、活化、抑制和增殖的作用情况。结果与结论:骨髓来源和脂肪来源的间充质干细胞同样具有抑制T细胞增殖的能力,在有丝分裂原刺激和混合淋巴细胞反应的T细胞增殖中这种作用都是具有剂量依赖性的,在1︰2时有极强的抑制作用,但是在1︰100时这种作用基本消失,在共培养时骨髓来源和脂肪来源的间充质干细胞都可以使更多的T细胞被抑制在G0/G1期,同时也可以抑制T细胞的早期活化,但是上述作用脂肪来源的间充质干细胞均较骨髓来源间充质干细胞弱,且脂肪来源的间充质干细胞并不具有抑制T细胞凋亡的作用。

BACKGROUND：Do adipose-derived mesenchymal stem cells（ADMSCs） have a similar role with bone marrow mesenchymal stem cells（BMSCs） in immune regulation？ OBJECTIVE：To observe the immunological characteristics of ADMSCs and BMSCs.METHODS：ADMSCs and BMSCs were isolated to detect T cell cycle,activation,inhibition and proliferation.RESULTS AND CONCLUSION：BMSCs and ADMSCs have the same function to inhibit T cell proliferation in mitogen-stimulated and mixed lymphocyte reaction of T cell proliferation in a dose-dependent manner：a strong inhibitory effect was found at a ratio of 1：2,but disappeared at a ratio of 1：100.Coculutre of BMSCs and ADMSCs could inhibit more T cells in the G0/G1 stage,and simultaneously inhibit the early activation of T cell.But the role of ADMSCs was less than that of BMSCs and ADMSCs could not inhibit T-cell apoptosis alone.