基质细胞衍生因子1α预处理内皮祖细胞移植治疗糖尿病下肢缺血

Endothelial progenitor cells pre-cultured with stromal cell derived factor 1 alpha transplantation to treat hind limb ischemia in diabetic rats

背景:基质细胞衍生因子1α被证明可以促进内皮祖细胞归巢。目的:观察基质细胞衍生因子1α预处理内皮祖细胞移植治疗糖尿病模型鼠下肢缺血的疗效。方法:取雄性Wistar鼠30只,25只成功建立糖尿病模型大鼠,随机数字表法分为3组,对照组注入等量生理盐水,共培养组注射与基质细胞衍生因子1α共培养的内皮祖细胞,内皮祖细胞组注入未进行共培养的内皮祖细胞。结果与结论:MTT检测结果显示,基质细胞衍生因子1α能显著增加内皮祖细胞的增殖能力(P<0.01)。移植后第28天动脉造影显示共培养组缺血侧下肢动脉显影血管数多于内皮祖细胞组和对照组(P<0.05)。提示采用基质细胞衍生因子1α预处理内皮祖细胞,有利于使糖尿病大鼠缺血下肢血供改善,主要来自于新生血管形成和/或原有细小血管的代偿性增粗。

BACKGROUND：Stromal cell derived factor 1 alpha（SDF-1α） has been proved to promote endothelial progenitor cells（EPCs） homing.OBJECTIVE：To explore the effects of SDF-1α pre-cultured EPCs from autologous bone marrow on hind limb ischemia in diabetic rat models.METHODS：Thirty male Wistar rats（successful model preparation in 25 rats） were randomly divided into 3 groups：normal saline control group（A group）,EPCs transplantation in alloxan-induced diabetes rats（B group）,EPCs cultured by SDF-1α transplantation in alloxan-induced diabetes rat（C group）.RESULTS AND CONCLUSION：MTT results showed SDF-1α could remarkably improve the proliferative ability of EPCs（P〈 0.01）.Angiography showed that angiogenesis index was significantly higher in the C group at the 28th day（P 〈0.05）.EPCs pre-cultured with SDF-1α have a promoted effect to reform the hind limb ischemia condition in diabetic rats;blood-supply mainly comes from new vessels and /or virgin minute blood vessel＇s compensation thickening.