期刊文献+

冷冻预处理温度对胎兔许旺细胞活性的影响 被引量:1

Effect of frozen pretreatment temperature on bioactivity of embryonic rabbit's Schwann cells
下载PDF
导出
摘要 背景:许旺细胞作为周围神经损伤修复的重要桥接材料,其活性对临床修复周围神经损伤成功与否具有重要影响。目的:观察不同维持温度冷冻预处理超深低温保存对胎兔许旺细胞活性的影响。方法:设计胎兔许旺细胞冷冻维持温度为-30~-70℃,每间隔-5℃为一组,以10%二甲基亚砜为低温保护剂,对胎兔许旺细胞进行上述温度冷冻预处理,液氮中保存48h,快速复温后继续培养48h,透射电镜观察细胞超微结构改变,MTT法和3H-TdR掺入法检测细胞活性。结果与结论:透射电镜下见-45℃处理的细胞超微结构良好,其余各温度组细胞均出现细胞器肿胀、坏死等轻重不同的冷冻损伤表现。MTT法和3H-TdR检测结果均显示-45℃冷冻预处理对许旺细胞的生长抑制率最低,分别为(3.83±0.56)%、(4.41±0.71)%,和其余各温度组之间相比,差异有显著性意义(P<0.05)。提示-45℃是胎兔许旺细胞冷冻预处理的最佳维持温度值,对细胞活性影响最小,可较好保持其生物学活性。 BACKGROUND:Schwann cells are considered as an important bridge material for peripheral nerve repair,and the activity of Schwann cells is a key to whether the repair of peripheral nerve injury succeeds or not.OBJECTIVE:To investigate the effect of different frozen pretreatment temperatures on the bioactivity of embryonic rabbit's Schwann cells preserved at ultra-low temperature.METHODS:The embryonic rabbit's Schwann cells were frozen at-30 ℃,-35 ℃,-40 ℃,-45 ℃,-50 ℃,-55 ℃,-60 ℃,-65 ℃ as well as-70℃ respectively,treated by a cryoprotectant,10% dimethyl sulfoxide,at the same time,preserved in liquid nitrogen for 48 hours,and cultured for 48 hours after rapid rewarming.Morphological changes of Schwann cells were observed under transmission electron microscopy(TEM).Bioactivity of Schwann cells was assessed by MTT and 3H-TdR incorporation test assays.RESULTS AND CONCLUSION:The ultrastructure of Schwann cells in the group of-45 ℃ remained intact under TEM;however,other experimental cells exhibited the different appearances of cryodamage such as swelling organelles,necrosis in the cytoplasm.Results in MTT and 3H-TdR assays showed that growth inhibiting rate in the group of-45 ℃ was(3.83±0.56)% and(4.41±0.71)%,which was the lowest among the experimental groups(P〈0.05).-45 ℃ is the optimal frozen pretreatment temperature that can protect the bioactivity of embryonic rabbit's Schwann cells.
出处 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2011年第36期6793-6796,共4页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 安徽省教育厅资助课题(2005KJ283)~~
  • 相关文献

参考文献6

二级参考文献86

共引文献24

同被引文献11

  • 1Kim IY, Seo SJ, Moon HS, et al. Chitosan and its deriva- tives for tissue engineering applications[J]. Biotechnol Adv, 2008, 26(1):1-21.
  • 2Yang YM, Hu W, Wang XD, et al. The controlling biodegra- dation of chitosan fibers by N-acetylation in vitro and in vivo[J]. J Mater Sci Mater Med, 2007, 18(11):2117-2121.
  • 3Aszmann OC, Korak K J, Luegmair M, et al. Bridging criti- cal nerve defects through an acellular homograft seeded with autologous Schwann cells obtained from a regenera- tion neuroma of the proximal stump[J]. J Reconstr Micro- surg, 2008, 24(3):151-158.
  • 4Gulati AK. Immunological fate of schwann cell-populated acellular basal lamina nerve allografts[J]. Transplantation, 1995, 59(11):1618-1622.
  • 5Wickware P. Progress from a fragile start[J]. Nature, 2000, 403(6768):466.
  • 6Farrant J, Knight SC, Mc Gann LE, et al. Optimal recovery of lymphocytes and tissue culture cells following rapid cooling[J]. Nature, 1974, 249(456):452-453.
  • 7Schaal SM, Kitay BM, Cho KS, et al. Schwann cell trans- plantation improves reticulospinal axon growth and fore- limb strength after severe cervical spinal cord contusion[J]. Cell Transplant, 2007, 16(3):207-208.
  • 8Azanchi R, Bernal G, Gupta R, et al. Combined demyeli- nation plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contu- sion injury[J]. J Neurotrauma, 2004, 21(6):775-788.
  • 9Laulas AA, Franeeschini I, Dubois-Dakg M, et al. Sehwann cells genetically engineered to express pSA show en- hanced migratory potential without impairment of their myelinating ability in vitro[J]. Glia, 2006, 53(8):868-878.
  • 10代秀松,肖玉周,吴敏,徐伟.冷冻预处理温度对胎兔许旺细胞免疫原性的影响[J].骨科,2011,2(1):17-19. 被引量:1

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部