新型脱细胞骨基质材料的制备及理化评估

Fabrication and characterization of a novel acellular bone matrix scaffold for bone tissue engineering

背景:传统的结构性天然骨脱细胞的方法存在许多不足之处。目的:用新的理化方法对结构性骨块进行脱细胞处理制作新型骨支架材料的可行性,并检测其理化性能。方法:以股骨头负重区结构性骨块为原料,高压水枪冲洗,继而利用Triton-100、脱氧胆酸钠等进行脱细胞等理化处理,制备新型脱细胞骨基质材料,并对支架进行大体、组织学、扫描电镜、Micro-CT观察、生物力学等相关检测。结果与结论:新型脱细胞骨基质材料保留了骨的细胞外基质成分,脱细胞彻底,扫描电镜及Micro-CT观察显示支架具备三维多孔网状结构系统,具有天然骨的孔径和孔隙率;生物力学测试脱细胞组支架的弹性模量为(552.56±58.92)MPa,强度为(11.34±3.49)MPa,与新鲜骨的弹性模量及强度比较,差异无显著性意义(P>0.05),可作为骨组织工程支架的良好载体。

BACKGROUND：Natural bone acellular cells have many inadequacies.OBJECTIVE：To fabricate and characterize a novel acellular bone matrix scaffold for bone tissue engineering,and to detect its physical and chemical properties.METHODS：Bone column was prepared using canine bone cancellous bone from the femoral head,washed with high-pressure water gun,and decellularized using TritonX-100 and sodium deoxycholatethe.The scaffold was investigated by histological staining,scanning electronic microscope observation,Micro-CT and biomechanical analysis.RESULTS AND CONCLUSION：The new bone scaffold showed most of the extracellular matrix components after removal of the cell fragments after decellularization.Scanning electron microscopy and Micro-CT revealed a three-dimensional interconnected porous structure,with the pore diameter and porosity of nature bone.The longitudinal elastic modulus of the new scaffold was（552.56±58.92） MPa,and the intensity was（11.34±3.49） MPa.There was no significant difference between the natural bone and the new scaffold（P 0.05）.The new acellular bone matrix scaffold is a good alternative cell-carrier for bone tissue engineering.