促血管内皮细胞生长的功能化自组装多肽框架材料的筛选和制备

Screen and synthesis of functionalized self-assembling peptide nanofiber scaffolds for promoting vascular endothelial cells growth

背景:功能化多肽框架材料由于良好的生物相容性及生物活性,可以用来促进血管生成的研究。目的:设计并筛选出能够促进内皮细胞血管生成的功能化多肽框架材料。方法:将设计和筛选出的功能多肽片断通过固相合成法复合在自组装多肽RADA16-I的C末端,将RADA16-I与功能化自主装多肽以1:1的比例混合,加入无菌培养板在37℃下孵育过夜以促进其凝胶,通过换培养基调节pH值。在凝胶上对内皮细胞进行2D培养。观察功能化自组装多肽框架材料的圆二色谱、原子力显微镜照像,内皮细胞黏附、增殖情况。结果与结论:功能化自组装多肽框架材料与Matrigel的形貌相似且是均一的纳米纤维材料。其中RAD/KLT和RAD/PRG具有促进内皮细胞黏附及增殖的功能。表明,功能化多肽框架材料RAD/KLT和RAD/PRG具有用于促内皮细胞血管生成的进一步研究的潜力。

BACKGROUND：Due to the unique biocompatibility and bioactivity,functionalized self-assembling peptide nanofiber scaffolds can be used to promote angiogenesis.OBJECTIVE：To design and screen the functionalized self-assembling peptide nanofiber scaffolds that can promote endothelial cell angiogenesis.METHODS：The designed and selected functionalized peptide sequences were directly extended on the self-assembling peptide RADA16-I through solid phase synthesis at the C-termini to increase the angiogenic activities of RADA16-I peptide scaffolds by CPC Scientific.The functionalized self-assembling peptide was mixed with RADA16-I at the ratio of 1：1.Then,the mixture was added into sterile culture plate and cultured at 37 ℃ overnight to form gel.pH value was mediated by changing culture medium.Human umbilical vascular endothelial cells were cultured on the surface of the peptide scaffolds for two-dimensional culture.The functionalized self-assembling peptide nanofiber scaffolds were characterized by circular dichroism spectrum and atomic force microscopy image.RESULTS AND CONCLUSION：The structure of functionalized self-assembling peptide nanofiber scaffolds is quite similar with Matrigel and they are uniformed nanofiber materials.These two designer functionalized peptides,KLT and PRG,have been demonstrated to significantly enhance endothelial cell adhesion,survival and rapid proliferation.The scaffolds of RAD/KLT and RAD/PRG can be used for the study of promoting endothelial cell angiogenesis.