三维支架材料与脂肪干细胞的生物相容性

Biocompatibility of three-dimensional scaffolds with adipose-derived stem cells

背景:构建组织工程化气管需要适合的三维支架。目的:观察脂肪干细胞与聚乳酸-乙醇酸共聚物及聚三亚甲基碳酸酯共聚物支架的生物相容性。方法:采用组织块法原代分离培养SD大鼠脂肪干细胞,行流式细胞术及多向分化能力鉴定。将脂肪干细胞分别种植于聚乳酸-乙醇酸共聚物和聚乳酸-乙醇酸-三亚甲基碳酸酯共聚物支架中,扫描电镜观察细胞与支架的生物相容性。结果与结论:脂肪干细胞种植于两种支架材料后生长速度快,扫描电镜观察可见脂肪干细胞呈球型,并伸展形成伪足,贴附于支架材料,细胞间相互连接成团。说明聚乳酸-乙醇酸共聚物与聚三亚甲基碳酸酯共聚物支架均具有良好的生物相容性,无细胞毒性,其多孔的三维立体状结构适合脂肪干细胞黏附生长。

BACKGROUND：An optimal three-dimensional scaffold is essential for construction of tissue engineered trachea.OBJECTIVE：To observe the biocompatibility of adipose-derived stem cells（ADSCs） with poly（lactic acid-glycolic acid） and poly（lactic acid-glycolic acid-trimethylene carbonate） scaffolds.METHODS：ADSCs were isolated and cultured by explant culture method.Cell surface markers were evaluated by flow cytometry and the multilineage differentiation ability was investigated.The biocompatibility of ADSCs with poly（lactic acid-glycolic acid） and poly（lactic acid-glycolic acid-trimethylene carbonate） scaffolds was observed by scanning electron microscopy.RESULT AND CONCLUSION：ADSCs were seeded into the two kinds of scaffolds and cultured in vitro.Scanning electron microscopy showed that ADSCs were spherical and well attached to the scaffolds with lamellipodia and conglobate connection.The poly（lactic acid-glycolic acid） and poly（lactic acid-glycolic acid-trimethylene carbonate） scaffolds with porous structure were highly biocompatible and noncytotoxic as determined by ADSCs adhesion and proliferation.