摘要
背景:采用PCR、ELISA、免疫荧光球法,以及基因芯片技术进行肠道病原菌的快速检测,需要数小时才能得到结果,不适用于现场快速检验。目的:制备聚丁二炔纳米粒,对大肠埃希氏菌进行免疫检测。方法:采用超声分散法制备聚丁二炔纳米粒,并利用其检测大肠埃希氏菌。结果与结论:制备的聚丁二炔纳米粒粒径均匀,分布范围窄,结构稳定。取大肠埃希氏菌稀释液滴入聚丁二炔免疫纳米粒稀释液中2min,溶液颜色由蓝变红,表明聚丁二炔纳米粒的检测速度快;可见吸收光谱图、透射电镜图、激光粒径分布图均显示,加入大肠埃希氏菌后聚丁二炔免疫纳米粒变化很大。在利用聚丁二炔免疫纳米粒比色检测大肠埃希氏菌的过程中,其鼠抗大肠埃希氏菌多克隆抗体起到了功能分子的作用,抗原-抗体结合反应是特异性的。
BACKGROUND:PCR,ELISA,immunofluorescence-ball method,and gene chip technology for rapid detection of enteric pathogens take hours to get results and are not suitable for on-site rapid test.OBJECTIVE:To prepare polydiacetylene(PDA) nanoparticles for immunodetection to Escherichia coli.METHODS:PDA nanoparticles were prepared with sonication.RESULTS AND CONCLUSION:The prepared PDA nanoparticles had uniform particle size,narrow distribution,and stable structure.Escherichia coli dilution was dropped into PDA nanoparticle dilution for 2 minutes,and the solution color turned from blue to red,indicating that PDA nanoparticles were rapid in detection speed.The graphs of ultraviolet-visible absorption spectroscopy,transmission electron microscope,and nanosizer displayed big changes with Escherichia coli and antibody PDA nanoparticle.The experimental results signed that the rat polyclonal antibody is a functional molecule of the immunodetection to Escherichia coli of PDA nanoparticles,and the reaction of antigen-antibody is specific.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第38期7105-7108,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
江西省教育厅科技项目(GJJ09359)
江西省科技支撑计划资助项目(2009BSA12400)~~