聚丁二炔纳米粒对大肠埃希氏菌的免疫检测

Immunodetection to Escherichia coli of polydiacetylene nanoparticles

背景:采用PCR、ELISA、免疫荧光球法,以及基因芯片技术进行肠道病原菌的快速检测,需要数小时才能得到结果,不适用于现场快速检验。目的:制备聚丁二炔纳米粒,对大肠埃希氏菌进行免疫检测。方法:采用超声分散法制备聚丁二炔纳米粒,并利用其检测大肠埃希氏菌。结果与结论:制备的聚丁二炔纳米粒粒径均匀,分布范围窄,结构稳定。取大肠埃希氏菌稀释液滴入聚丁二炔免疫纳米粒稀释液中2min,溶液颜色由蓝变红,表明聚丁二炔纳米粒的检测速度快;可见吸收光谱图、透射电镜图、激光粒径分布图均显示,加入大肠埃希氏菌后聚丁二炔免疫纳米粒变化很大。在利用聚丁二炔免疫纳米粒比色检测大肠埃希氏菌的过程中,其鼠抗大肠埃希氏菌多克隆抗体起到了功能分子的作用,抗原-抗体结合反应是特异性的。

BACKGROUND：PCR,ELISA,immunofluorescence-ball method,and gene chip technology for rapid detection of enteric pathogens take hours to get results and are not suitable for on-site rapid test.OBJECTIVE：To prepare polydiacetylene（PDA） nanoparticles for immunodetection to Escherichia coli.METHODS：PDA nanoparticles were prepared with sonication.RESULTS AND CONCLUSION：The prepared PDA nanoparticles had uniform particle size,narrow distribution,and stable structure.Escherichia coli dilution was dropped into PDA nanoparticle dilution for 2 minutes,and the solution color turned from blue to red,indicating that PDA nanoparticles were rapid in detection speed.The graphs of ultraviolet-visible absorption spectroscopy,transmission electron microscope,and nanosizer displayed big changes with Escherichia coli and antibody PDA nanoparticle.The experimental results signed that the rat polyclonal antibody is a functional molecule of the immunodetection to Escherichia coli of PDA nanoparticles,and the reaction of antigen-antibody is specific.