甲状腺素对阿尔茨海默病患者淋巴细胞糖原合酶激酶-3的影响

The Effects of Thyroid on Glycogen Synthase Kinase-3 Activity in Lymphocytes in Alzheimer Disease

目的:探讨甲状腺素对阿尔茨海默病患者淋巴细胞糖原合酶激酶-3(glycogen synthase kinase-3,GSK-3)的影响。方法:用^(32)P标记法检测正常老年人组,阿尔茨海默病(Alzheimer's disease,AD)组,甲状腺素治疗组淋巴细胞糖原合酶激酶-3的变化,并用放射免疫法检测这些组血清三碘甲状腺原氨酸(triiodothyronine,T_3)、T_4甲状腺素(tetraiodothyronine,T_4)、游离三碘甲状腺原氨酸(freetriiodothyronine,FT_3)、T_4游离甲状腺素(free thyroxine,FT_4)、促甲状腺激素(thyrotropin,TSH)的变化。结果:与对照组比较,AD组及甲状腺素组治疗前T_3及FT_3的含量存在明显降低(P<0.01,P<0.01)。TSH水平也明显降低(P<0.01,P<0.01),且伴有淋巴细胞GSK-3活性的明显降低(P<0.01,P<0.01)。甲状腺素治疗后,T_3、FT_3、TSH水平明显恢复(P<0.01),淋巴细胞GSK-3的活性明显降低(P<0.01),与对照组无明显差别(P>0.05)。结论:AD患者甲状腺功能降低,且伴有GSK-3活性升高,甲状腺素治疗后甲状腺功能恢复,且GSK-3活性降低,提示甲状腺素对AD治疗可能有价值。

Objective：our pruepose was to explore the effects of thyroid on glycogen synthase kinase-3activity in lymphocytes in alzheimer disease.methods：the activity of GSK-3 was measured ty 32^p liquild scintillography for incorporated radioactivity,and the levels of serum triiodothy romine（T3） ,teraiodothyronine（T4）,free triiodotyronine（FT1）,free thyroxine（FT4）,and thyrotropin（TSH）were measured by radioimmunoassay in control group.Alzheimer disease group and thyroid treament group.Results：Compared with control group,the levels of T3、FT3and TSH were lower in AD group and in thyroid group unpretreatment（P〈0.01,P〈0.01）,moreover,GSK-3 activity was also increased in these two groups（P〈0.01,P〈0.01）,The higher levels of T3,FT3 and TSH were induced in thyroid treatment grop（P〈0.01）,and the activity of GSK-3 activity in AD group,thyroid function was recoveried and low level of GSK-3 activity was reversed after thyroid treatment. Out results suggust that thyroid be valuble for AD treatment.