90株多重耐药鲍曼不动杆菌耐药的分子机制研究

Study on the Molecular Mechanisms of 90 Multidrug-resistance Acinetobacter Baumannii Isolates

目的:研究临床分离的90株多重耐药鲍曼不动杆菌耐药分子机制。方法:用PCR检测β-内酰胺酶基因、Ⅰ型整合子基因和外排泵基因(adeB),并对扩增出的基因测序比对。结果:90株鲍曼不动杆菌,检出10株携带OXA-23基因,4株携带TEM基因,38株携带PER-1基因,56株携带外排泵基因(adeB);45株携带Ⅰ型整合子结构基因。结论:Ⅰ型整合子基因及β-内酰胺酶类基因的作用可能是导致鲍曼不动杆菌多重耐药和高耐药的两个重要原因,某医院流行产OXA-23型酶的菌株,需要采取措施防止其进一步传播。

Objective：To study the molecular mechanisms of 90 multidrug-resistance Acinetobactcr baumannii isolates. Methods：The -β-lactamase gene,class 1 integron gene and adeB gone were determined by PCR and Were then sequenced.Results：OXA-23 gene was detected positive in six A.baumanii isolates,which were all resistant to meropenem （MEM） and imipenem （IPM）.TEM and PER gene were detected positive in 4 and 38 strains,respectively.Gene adeB was positive in 56 strains,and class I integron gene was positive in 45 strains.Conclusion：Class 1 mtegron and β -lactamaso gene might be the causes of mutidrug-resistance and high resistance er A. baumani.Effective measures should be taken to control the epidemic of the strain With OXA-23 in one hospital.