罗格列酮对肥胖患者脂肪细胞中脂联素表达的影响

Effects of rosiglitazone on adiponectin expression in cultured adipocytes of obese patients

目的:探讨噻唑烷二酮类药物(thiazolidinediones,TZDs)罗格列酮(rosiglitazone,RSG)对肥胖患者脂肪细胞中脂联素表达的影响。方法:依据检查者的体质量指数(BMI)分为正常体重者和肥胖者,各3例。脂肪组织采用整形外科吸脂手术(供者知情并同意)获得。分离培养6例检查者的前脂肪细胞,用胰岛素、地塞米松、三碘甲状腺原氨酸(T3)及3-异丁基-1-甲基黄嘌呤(IBMX)诱导其分化为成熟的脂肪细胞。实验分为对照组和RSG处理组,前者培养的脂肪细胞不进行处理;后者的脂肪细胞用10 mol/L的RSG分别处理12 h、24 h、48 h和72 h。收集细胞的培养上清液,用ELISA法检测脂联素蛋白分泌的水平。提取细胞的总RNA,用RT-PCR法检测脂联素mRNA表达的水平。结果:肥胖患者成熟脂肪细胞脂联素mRNA及其蛋白表达的水平较正常体重者明显降低(P<0.05)。与对照组相比较,RSG组成熟脂肪细胞脂联素mRNA和其蛋白表达的水平在处理不同时间(12 h、24 h、48 h和72 h)后均明显增高(P<0.05或P<0.01),且呈时间依赖性;但肥胖患者脂联素mRNA和其蛋白表达水平增高持续的时间较正常体重者短(P<0.05或P<0.01)。结论:RSG在一定程度上能增加肥胖患者成熟脂肪细胞中脂联素表达的水平。

AIM：To investigate the effects of rosiglitazone （RSG） on adiponectin expression in cultured adipocytes of obese patients. METHODS： Subjects were divided into two groups： normal body weight and obese subjects according to body mass index. Adipose tissue was obtained after informed consent from adult patients undergoing elective liposuction in the Department of Plastic Surgery. Human preadipocytes were isolated and cultured for several days and then induced to differentiate into adipocytes with special culture medium supplemented with insulin, dexamethasone, T3 and IBMX. Adipocytes were randomly divided into control group and RSG group. Adipocytes in RSG group were then treated with RSG at a final concentration of 10 mol/L for different time durations （12, 24, 48 and 72 h）. Adiponectin concentrations in the supernatant were measured by ELISA, and the adiponectin mRNA expression of adipocytes was tested by RT-PCR. RESULTS： Adiponectin mRNA expression and protein secretion were significantly decreased in obese patients compared with patients of normal body weight （P〈0.05）. Compared with control group, adiponectin mRNA expression and protein secretion in RSG group significantly increased in a time-dependent manner （P〈0.05 or P〈0.01）. However, duration of the increase in obese patients was shorter than in patients of normal body weight （P〈0.01）. CONCLUSION： To some extent, RSG increases adiponectin expression in cultured adipocytes of obese patients.