组蛋白去乙酰化酶1蛋白在早期自然流产绒毛和蜕膜组织中的表达

Expression of HDAC1 protein in chorionic villi and decidua of early spontaneous abortion

目的:探讨组蛋白去乙酰化酶1(HDAC1)蛋白在早期自然流产发生中的病理生理作用及临床意义。方法:应用免疫组织化学和Western blot法检测早期自然流产和早期正常妊娠者绒毛及蜕膜组织中HDAC1蛋白的表达。结果:免疫组化示:HDAC1主要定位于绒毛细胞滋养细胞、合体滋养细胞和子宫蜕膜细胞、腺上皮细胞的胞核;早期自然流产绒毛组织中HDAC1的表达强度低于早期正常妊娠组,而蜕膜组织中,早期自然流产组织HDAC1的表达强度高于正常妊娠组,差异均有统计学意义(P均<0.05)。Westernblot示:早期自然流产绒毛组织中HDAC1蛋白的相对表达量为1.38±0.63,明显低于正常妊娠组(1.92±0.82),差异有统计学意义(P<0.05);而早期自然流产蜕膜组织中HDAC1蛋白的相对表达量为1.02±0.53,明显高于正常妊娠组(0.71±0.36),差异有统计学意义(P<0.05)。结论:HDAC1在早期自然流产绒毛和蜕膜组织中的异常表达可能在早期自然流产的发生、发展中起重要作用。

Objective：To study the pathophysiological roles and clinical significance of HDAC1 protein in chorionic villi and decidua of patients with early spontaneous abortion.Methods：The expression of HDAC1 in chorionic villi and decidua of early spontaneous abortion and normal pregnancy was detected using immunohistochemistry and Western-blot.Results：Immunoreactive staining of HDAC1 was predominantly located in the nuclei of syneytiotrophooblast,cytotrophoblast cell of chorionic villi,and decidual cell,glandular epithelium cell of decidua.The intensity of HDAC1 staining in the chorionic villi of early spontaneous abortion was lower than that of normal pregnancy,while the intensity of HDAC1 staining in decidua was higher than that of normal pregnancy,and the differences were significant（P0.05）.Western-blot showed that HDAC1 protein level in the chorionic villi of early spontaneous abortion was significantly lower than that of normal pregnancy（1.38±0.63 vs 1.92±0.82,P0.05）.HDAC1 protein level in the decidua of early spontaneous abortion was significantly higher than that of normal pregnancy（1.02±0.53 vs 0.71±0.36,P0.05）.Conclusion：Abnormal expression of HDAC1 in the chorionic villi and decidua may play an important role in the process of early spontaneous abortion.