输卵管妊娠人输卵管黏膜上皮VDR的表达

Expression of vitamin D receptor (VDR) in human tubal epithelium of tubal pregnancy

目的:探讨输卵管妊娠病理状态下人输卵管黏膜上皮VDR蛋白在受精卵种植部位与非种植部位的表达及其变化。方法:应用免疫组织化学方法检测14例人输卵管妊娠手术切除标本的黏膜上皮细胞VDR蛋白的表达。结果:输卵管妊娠种植部位和非种植部位输卵管黏膜上皮均可检测到VDR蛋白表达,VDR蛋白阳性于输卵管黏膜上皮细胞的细胞核内表达,部分间质平滑肌细胞核内亦可见阳性染色。种植部位输卵管黏膜上皮细胞VDR蛋白表达(5.95±1.81)明显少于非种植部位(7.55±3.20)(P=0.017,P<0.05),有些部位VDR蛋白表达甚至缺如。结论:人输卵管黏膜上皮VDR蛋白表达的改变可能是输卵管妊娠的发病原因之一。

Objective：To detect the expression and change of vitamin D receptor（VDR）protein in the human tubal mucosal epithelium of tubal pregnancy,and analyze the potential relationship between the VDR expression and the occurrence of tubal pregnancy.Methods：The expression of VDR protein in the human tubal mucosal epithelium in both implantation and nonimplantation sites of tubal pregnancy was detected by immunohistochemistry.Results：The expression of VDR protein was detected in the human tubal mucosal epithelium in both implantation and nonimplantation sites of tubal pregnancy.The VDR positive staining was mainly located in the nucleus of the epithelial cells,occasionally in some smooth muscle cells.The VDR positive expression of the human tubal mucosal epithelium was in a low level in implantation site,however,increased in nonimplantation site significantly（P=0.017,P0.05）.Conclusions：The change in expression of VDR protein in the human tubal mucosal epithelium would be a possible cause of ectopic pregnancy.