三维培养的牙髓干细胞参与牙髓损伤修复能力的实验研究

Effect of dental pulp stem cell cultured by three dimensions on reparation of dental injury

目的:将体外建立的细胞因子BMP-2作用下牙髓干细胞(DPSC)三维培养体系应用于大鼠牙髓损伤模型中,观察DPSC对牙髓组织损伤修复的能力。方法:已构建的三维培养DPSC的细胞团添加BMP-2体外持续培养7 d,BrdU标记及检测鉴定后,将其置于建立的大鼠牙髓损伤模型洞内,移植4周后HE染色,改良Mallory三色法染色观察,免疫组化检测BrdU标记的细胞分布。结果:移植后4周,DPSC三维细胞培养的牙髓盖髓实验中有骨样牙本质基质形成,没有观察到组织的炎症和坏死。在基质中可以看到骨性成牙本质样细胞,带有长的突起的成牙本质样细胞在骨性牙本质中形成管状牙本质。结论:损伤的牙髓表面植入添加BMP处理的DPSC细胞团能够产生修复性牙本质。

Objectine：In the rat dental injury model,3-dimension culture system of dental pulp stem cell（DPSC） affected by BMP-2 was used.The ability of DPSC to the dental injury was observed.Method：The DPSC mass in the 3-dimension culture system was cultured for 7 days after added with BMP-2.The cell mass was marked and identified by Brdu,then was put in the holes of rat dental injury model.Result：Osteoid dentin substance was found in the 3-dimension culture system of DPSC after 4 weeks.No tissue inflammation and necrosis was found..Osteoid dentium cell could be seen in the substance.Dentium cell with high apophysis was developed to the tubiform dentium in the osteoid dentin substance.Conclusion：Injured dental surface implanted by the DPSC mass added with BMP can bring about the reparative dentin.