山羊下颌骨三焦点牵张成骨的超微结构观察

The research in the ultrastructure in goats′mandible of trifocal distraction osteogenesis

目的:为探索三焦点牵张成骨的成骨方式及新骨改建过程提供实验依据。方法:取6只山羊下颌骨牵张成骨形成的新生骨组织及其邻近原骨组织,硬组织磨片,扫描电镜观察骨组织断面的超微结构,同时用硬组织磨片四环素荧光双标记技术分析。结果:牵张间隙超微结构观察显示大量新生骨小梁,骨质密度好,中央区新生类骨质沿牵张方向排列,新骨与原骨边界呈骨性融合,在牵张区内有大量的荧光结合,并与周围原有组织有明显的界限。结论:山羊下颌骨三焦点牵张成骨形成的新生骨段可逐渐改建为具有正常结构的骨组织。

Objective：For reaserching the ossification of the trifocal distraction osteogenesis and the progress of the new bone＇s reconstructing to provide the experiment material.Method：The samples were taken from the bone tssue of neogenesis and original one which made by 6 goats＇ trifocal distraction osteogenesis experiment.The cutting hard tissue of that was watched by SEM to observe the ultrastructure of the fracture serface.And we have the analysis by using the tetracycline fluorescence paired labeled technique.Result：The ultrastructure showd large measure bone trabecula of neogenesis.The density of the bone was great.The bone of central neogenesis arrange follow the direction of distraction.The edge between the original and the fresh area of bone have mix together.The incorporation of fluorescence could found in the area of involving.And it have the obvious edge between the original bone around.Conclusion：The new bone made by the trifocal distraction osteogenesis could be rebuilt to regular bone tissue gradually.