用免疫信息学技术设计新型H1N1流感神经氨酸酶抗原表位肽(英文)

Characterization of Epitope Peptides of Neuraminidases of The 2009 A H1N1 Viruses Designed by Immunoinformatics

神经氨酸酶(NA)是一种具有唾液酸酶活性的流感毒粒表面糖蛋白,切断病毒HA与细胞膜上神经氨酸残基之间的连接,使病毒能够从宿主细胞表面释放.检测了新型H1N1流感NA基因核苷酸序列,用免疫信息学方法预测、筛选和鉴定B细胞表位;人工合成NA抗原多肽SE8和RE6,并免疫新西兰兔制备抗血清.两种抗血清具有体外中和新型H1N1流感病毒能力(微量中和实验),而且还具有拮抗血凝释放作用.根据2009年全球毒株NA基因序列检测和比对结果,发现多肽SE8和RE6序列未变异.实验揭示,多肽SE8和RE6可以作为新型H1N1流感候选多肽疫苗.

The neuraminidase （NA） of influenza virus is a receptor-destroying enzyme, removing sialic acid from carbohydrate chains attached to HA, and releasing the viruses from infected cells. The NA genes of the 2009 A H1N1 were sequenced, then the B-cell epitopes were predicted, screened and assessed based on immunoinformatics. Two peptides SE8 and RE6 （amino acid residues 168-175 and 428-433） of NA protein were synthesized and immunized to raise antisera in rabbits. Two antisera are capable of eliciting neutralizing antibodies against 2009 A H1N1 in the in vitro microneutralization assay, furthermore, the anti-releasing effects of hemagglutination existed in the antisera. Alignment with databases showed that the amino acid residues of two epitope peptides are highly conserved amongst the NA sequences of the strains isolated from the world. These findings indicate that SE8 and RE6 represents an attractive candidate for an effective synthetic peptide-based vaccine against 2009 A H 1N 1 viruses.