核呼吸因子-1和Ki-67在乳腺癌组织中的表达及意义

Expression of Nuclear Respiratory Factor-1 and Ki-67 in Breast Cancer and Its Significance

目的：探讨核呼吸因子-1（NRF-1）蛋白表达与乳腺癌发生发展、临床病理特征及Ki-67的关系.方法：采用免疫组化EnVision二步法,对211例乳腺癌组织、50例乳腺良性病变组织中NRF-1和Ki-67进行检测.结果：NRF-1表达定位于细胞核,在乳腺癌中表达率为87.2%,低于乳腺良性病变组织的100%（χ2=100.288,P〈0.001）;高分化乳腺癌NRF-1表达率明显高于中、低分化乳腺癌（χ2=16.242,P=0.001;χ2=72.802,P〈0.001）中分化乳腺癌也明显高于低分化乳腺癌（χ2=30.190,P=0.000）.乳腺癌淋巴结转移患者NRF-1表达率明显低于未转移者（χ2=12.0,25,P=0.007）;TNM分期中I期NRF-1表达率明显高于Ⅱ、Ⅲ期（χ2=12.025,P=0.007）.211例乳腺癌中Ki-67表达率为78.7%,Ki-67表达与NRF-1（χ2=42.914,P〈0.001）、病理组织学分级（χ2=40.239,P〈0.001 ）、淋巴结转移（χ2=16.061,P=0.001）和TNM分期（χ2=13.589,P=0.004）间比较差异有统计学意义.结论：NRF-1参与了乳腺癌的发生发展,也与Ki-67表达有相关性,可作为乳腺癌患者疾病进展监控和预后观测的指标。

Objective： To investigate the correlation among the nuclear respiratory factor-1 （ NRF-1 ） expression and the carcinogenesis, invasion, metastasis, as well as Ki-67 expression, in breast cancer. Methods： NRF-1 and Ki-67 expression in 211 cases with breast cancer and 50 with benign lesion of the mammary gland were determined with immunohistochemical EnVision indirect method. Results： NRF-1 was located at the nucleus, The positive rates of NRF-1 expression were 82.7% in breast cancer and 100% in benign lesion of the mammary gland （ x^2 = 100.288, P = 0.000 ）. NRF-1 expression rates were significantly higher in well-differentiated breast cancer than those in the moderately and poorly differentiated breast cancers （ x^2 = 16.242, P = 0.001; 22 = 72.802, P = 0.000, respectively ）. In addition, NRF-1 expression was higher in the moderately differentiated breast cancer than in the poorly differentiated breast cancer （x^2 = 30.190, P = 0.000 ）. NRF-1 expression was lower in the patients with nodal metastasis than that in patients without a similar condition （ x^2 = 12.025, P = 0.007）. The positive rate of NRF-1 was significantly lower in the TNM II and III stages than in the TNM I stage （ X^2 = 12.025, P = 0.007）. The positive rate of Ki-67 was 78.7% in 211 patients with breast cancer. There were significant differences among the Ki-67 and NRF-1 expression rates （ X^2 = 42.914, P= 0.000）, pathohistological grade （ Z2 = 40.239, P= 0.000）, nodal metastasis （ X^2 = 16.061, P= 0.001）, and TNM staging （ x^2 = 13.589, P= 0.004）. Conclusion： NRF-1 expression plays an important role in the occurrence, as well as development, of breast cancer and correlates with Ki-67 expression. NRF-1 expression can be a marker in the progression and prognosis of breast cancer and may be used as the target for clinical treatment.