没食子鞣质,1,3,4-三-O-没食子酰基-6-O-咖啡酰基-β-D-吡喃葡萄糖,抑制人肝癌HepG_2细胞的增殖和调控miRNA的表达(英文)

1,3,4-tri-O-galloyl-6-O-caffeoyl-β-D-glucopyranose,a new anti-proliferative ellagitannin,regulates the expression of microRNAs in HepG_2 cancer cells

目的 miRNA在细胞的增殖,分化和凋亡中起重要作用。1,3,4-三-O-没食子酰基-6-O-咖啡酰基-β-D-吡喃葡萄糖(BJA32515)是从日本蛇菰中分离的天然多酚化合物,该化合物对肿瘤细胞的增殖和miRNA的影响未见报道。本文旨在研究BJA32515对人肝癌细胞HepG2增殖的抑制作用以及对miRNA表达的影响。方法采用CCK-8的方法检测HepG2细胞的增殖;用流式细胞法检测HepG2细胞的凋亡;采用miRNA芯片分析方法测定HepG2细胞的miRNA表达以及用RT-PCR的方法验证miRNA的表达。结果 BJA32515以时间和剂量依赖的方式抑制HepG2细胞的增殖,并促进细胞的凋亡。miRNA芯片结果显示,BJA32515能诱导HepG2细胞33个miRNA的表达上调以及59个miRNA的下调。RT-PCR结果也证实BJA32515可诱导let-7a和miR-29a的上调以及miR-373和miR-197的下调,与芯片分析的结果一致。结论BJA32515抑制HepG2细胞增殖的作用机制与miRNA的表达调控有关。

Background MicroRNAs（miRNAs） play important roles in cell proliferation,differentiation and apoptosis.1,3,4-tri-O-galloyl-6-O-caffeoyl-β-D-glucopyranose（BJA32515） is a new natural ellagitannin compound extracted from Balanophora Japonica MAKINO.The effect of BJA32515 on the expression of miRNAs in cancer cells has not yet been explored.Objective The present study was carried out to examine the changes in miRNA expression profiles in human HepG2 hepatocarcinoma cells following BJA32515 exposure.Methods The proliferation of BJA32515-exposed HepG2 cells was assessed using a colorimetric assay（cell counting kit-8）.The miRNA expression profile of the cancer cells was analyzed using a miRNA array and quantitative real-time PCR.Apoptosis was assessed by annexin V and propidium iodide staining.Results BJA32515 inhibited the cell proliferation and increased apoptosis in HepG2 cancer cells.The exposure to BJA32515 also caused alterations in the miRNA expression profile in the cells,with 33 miRNAs upregulated and 59 down-regulated.The up-regulation of let-7a and miR-29a and the down-regulation of miR-373 and miR-197 were verified by quantitative real-time PCR.Conclusion BJA32515-modifed miRNA expression may mediate the antiproliferative effect of this compound in HepG2 cancer cells.