大鼠胚胎卵巢原位移植的生殖内分泌功能研究

Recovery of reproductive endocrine function after orthotopic fetal ovarian allotransplantation in rats

目的建立大鼠胚胎卵巢原位移植模型,探讨大鼠胚胎卵巢原位移植后的生殖内分泌功能恢复情况,为进一步研究其生育和子代健康安全性提供前期基础。方法将90只日龄50~60 d的雌性SD大鼠随机分为移植受体组（研究组）50只、正常对照组（阳性对照组）20只、去势对照组（阴性对照组）20只。研究组行孕17~19 d胎龄胚胎卵巢原位移植（同时切除自身卵巢）、阳性对照组仅行开关腹手术、阴性对照组行自身卵巢切除而不行胚胎卵巢移植。于术后45 d随机取研究组、阳性和阴性对照组大鼠分别10只,共30只（其余60只雌性大鼠继续饲养供后期实验用）,采用电发光方法检测各组大鼠血清雌、孕激素水平,并同时剖腹探查卵巢发育情况、切除卵巢行病理切片HE染色观察卵泡发育情况。结果（1）移植术后雌孕激素恢复情况：移植后45 d,研究组、阳性和阴性对照组（每组各10只雌性大鼠）的雌激素分别为（114.65±15.59）、（121.71±14.92）和（56.51±7.82）pmol/L,孕激素分别为（12.79±4.40）、（13.00±1.18）和（3.70±0.68）ng/ml。研究组和阳性对照组间雌孕激素水平比较无显著性差异（P〉0.05）,而研究组和阴性对照组间雌孕激素水平比较有显著性差异。（P〈0.05）。（2）移植后卵巢发育情况：移植后45 d,研究组、阳性和阴性对照组的卵巢体积分别为（0.22±0.07）、（0.24±0.09）和0ml,研究组、阳性对照组间卵巢容积比较无显著性差异（P〉0.05）。光镜下研究组和阳性对照组卵巢内均可见不同阶段发育的卵泡和黄体形成。结论大鼠胚胎卵巢原位移植术后的卵巢可以存活,并可生长发育而具有排卵功能,其生殖内分泌功能可以恢复。

Objective To assess the recovery of the reproductive endocrine function in rats following orthotopic transplantation of fetal ovarian allograft.Methods Ninety female SD rats（50-60 days old） were randomized into graft recipient group（n=50）,positive control group（n=20）,and negative control group（n=20） to receive orthotopic transplantation of fetal（17-19 gestational days） ovaries following bilateral oophorectomy,sham abdominal surgery,and bilateral oophorectomy,respectively.At 45 days after the surgeries,serum estradiol and progesterone levels were measured and the ovaries were removed for evaluation of the ovarian volume and follicle development.Results On day 45 after the operations,the estradiol or progesterone levels showed no significant difference between the recipient group and positive control group（P0.05）,but both were significantly lowered in the negative control group（P0.05）.The ovarian volume was comparable between the recipient group and positive control group（P0.05）,and optical microscopy showed follicles in different stages of development and formation of corpus luteum in the ovaries in both groups.Conclusion Fetal rat ovary allografts can develop into functional ovaries capable of ovulation to restore the reproductive endocrine function of recipient female rats.