雷奈酸锶对大鼠骨质疏松性骨折愈合的骨密度及骨微结构影响

The effect of strontium ranelate on bone mineral density and bone microstructure in rat osteoporotic fracture healing

目的观察雷奈酸锶对大鼠胫骨骨质疏松性骨折愈合过程中的骨密度、骨微结构及愈合后生物力学特性的影响。方法 72只雌性SD大鼠卵巢去势制造骨质疏松模型,造模成功后制造开放性骨折并予克氏针内固定。术后分为对照组和治疗组,分别给予生理盐水和雷奈酸锶625mg/(kg.d)灌胃给药。结果在治疗后6、8w时两组骨性愈合率并无明显差异,但Micro CT检验数据结果显示治疗组骨密度(BMD);骨小梁体积(BV)、组织体积(TV)、骨体积分数(BV/TV)、平均骨小梁厚度(Tb.Th)、平均骨小梁数目(Tb.N)均明显高于对照组(P<0.05),且治疗组最大压缩荷载在治疗后4w(48.1±5.6(治疗组)vs 21.8±4.8(对照组))、8w时亦明显高于对照组(96.5±9.7(治疗组)vs68.2±7.4(对照组))。结论雷奈酸锶能够抑制骨折后的骨量丢失,增加骨密度,促进骨折愈伤组织的生长,改善骨小梁三维结构及骨组织的力学性能,可用来促进骨质疏松性骨折的愈合并改善骨质量,预防再骨折。

Objective This study was designed to investigate the influence of strontium ranelate in bone mineral density （BMD）, bone microstructure, and bone biomeehanieal characteristics during the bone healing after osteoporotic fracture in rats. Methods Seventy-two SD rats were ovariectomized to establish osteoporosis model. They were then performed open fracture and fixed with Kirschner＇s needle. After the surgery, rats were divided into treatment group （treated with strontium ranelate 625mg/kg/day） and control group （ lavaged with normal saline）. Results The bone healing was not different between the two groups 6- and 8-week after the surgery. According to micro-CT results, BMD, bone volume （BV） , tissue volume （ TV）, bone volume ratio （ BV/TV）, trabecular thickness （Tb. Th）, and trabecular number （Tb. N） in the treatment group were significantly higher than those in control group. The maximal loadings in the treatment group were significantly elevated than those in control group on 4 weeks （48.1 ± 5.6N vs 21.8 ±4.8N） and 8 weeks （96.5 ±9.7N vs 68.2 ±7.4N） after the surgery. Conclusions Strontium rauelate can inhibit bone loss after bone fracture. It can increase BMD, stimulate fracture-healing tissue growth, improve trabecular 3D structure and biomechanical strength. It can be applied in stimulating osteoporosis bone fracture healing, improving bone quality, and preventing of further fracture.