传统中药提取物对小鼠光老化皮肤中SOD活力与MDA含量的影响

Effect of extracts from four Chinese traditional medicines on SOD and MDA in photoaging BALB/c mice skin

目的:研究甘草等提取物对中波紫外线(UVB)照射BALB/c小鼠光老化皮肤组织中丙二醛(MDA)及超氧化物岐化酶(SOD)含量的影响。方法:分别将5%甘草、红景天、党参及黄芪提取物溶液涂于小鼠背部皮肤,20 min后给予500 mJ/cm2UVB辐射,1次/d×1月。取照射部位皮肤,测定SOD活力及MDA含量。结果:与空白对照组相比,UVB组中SOD活力显著降低,MDA含量显著增高(均P<0.01);与紫外线照射组相比,5%甘草、红景天、党参、黄芪提取物溶液组均可使SOD显著增高,MDA显著降低(均P<0.01);其中以甘草组最为明显,各药物组间相比有统计学差异(P<0.01)。结论:UVB慢性辐射可导致皮肤光老化,而甘草等提取物局部外用可能通过提高皮肤组织中SOD活力,降低MDA含量而较好的预防光老化及抗氧化作用。

Objective：To study the effect of extracts from four Chinese traditional medicines （Radix Glycyrrhi- za, Radix Rhodiola Crenulata, Radix Codonopsis Pilosulae, Radix Astragali ） on SOD and MDA in photoaging BALB/c mice skin. Methods： 5% water solution of four medicine extracts were used on the shaving area of mice, respectively. After 20 minutes, 500mJ/cm^2 UVB was irradiated per day. At the end of 1 month, skin samples were obtained and the values of SOD and MDA were measured. Results： SOD activity was statistically decreased, and MDA was remarkably increased in ultraviolet group compared with the blank control group （ P 〈 0.01 ） ; Mean- while, four medicines could significantly increase SOD activity and reduce MDA content （ P 〈 0.01 ）. Conclusion ： Extracts of Radix Glycyrrhiza, Radix Rhodiola Crenulata, Radix Codonopsis Pilosulae, Radix Astragali could enhance SOD activity and reduce MDA content in photoaging skin.