神经微丝蛋白免疫荧光染色法观察运动终板

Neurofilament immune fluorescence staining:a new method for observing the morphology of the motor endplate

目的:了解应用神经微丝蛋白免疫荧光染色法观察运动终板的效果。方法:取SPF级大鼠6只,麻醉状态下取材双侧比目鱼肌神经入肌点组织,以多聚甲醛固定,冰冻切片,行神经微丝蛋白免疫荧光染色,荧光显微镜和激光共聚焦显微镜下观察运动终板形态,并用激光共聚焦显微镜软件对运动终板形态进行三维重建。结果:染色切片在荧光显微镜和激光共聚焦显微镜下均可观察到运动终板爪形结构形态,激光共聚焦显微镜采集图像进行三维重建后,可以得到运动终板立体结构。结论:神经微丝蛋白免疫荧光染色法是一种较好的运动终板的观察方法。

Objective：To investigate the effect of neurofilament（NF） immune fluorescence staining on observing morphology of the motor endplate.Methods： Six SPF rats were used and their bilateral soleus muscles harvested under anesthesia.Then the samples were fixed with polyphosphate formaldehyde,and made frozen sections.Finally the neurofilament immunofluorescence staining was performed.The morphology of the motor endplate was observed under fluorescence microscope and laser scanning confocal fluorescence microscope.Results： The claw-shape motor endplate was seen clearly under the fluorescent microscope and laser scanning confocal fluorescence microscope,and the images observed used to reconstruct the three-dimensional structure of motor endplate.Conclusion： Neurofilament immune fluorescence staining is a useful method for the morphology study of the motor endplate.