星形孢菌素对前列腺癌细胞株PC-3增殖和凋亡的影响

Effects of staurosporine on the proliferation and apoptosis of prostate cancer PC-3 cells

目的:探讨蛋白激酶C抑制剂星形孢菌素(ST)对前列腺癌细胞株PC-3增殖及凋亡的影响。方法:以ST(10-8mol/L)处理体外培养的PC-3细胞株。采用Western印迹检测细胞周期蛋白cyclin A、cyclin D1表达的变化。通过MTT实验、平板克隆实验检测ST对PC-3细胞增殖能力的影响。流式细胞仪检测各组细胞凋亡的情况。光镜观察细胞形态学的改变。结果:PC-3细胞经ST处理后cyclin A、cyclin D1蛋白表达明显降低。MTT实验结果显示ST对PC-3细胞的抑制作用自48 h后(19.35%)有显著性(F=31.06,P<0.01)。平板克隆实验结果显示组细胞克隆形成率ST组[(37.10±3.43)%]明显低于对照组((64.80±4.34)%,χ2=14.59,P<0.05]及DMSO组[(62.80±4.36)%,χ2=12.50,P<0.05]。流式细胞术结果显示ST组凋亡率(19.6±2.20)%与对照组(5.33±1.40)%和DMSO组(5.50±0.96)%比较显著增加(F=104.36,P<0.01)。光镜下可见:与对照组及DMSO组比较ST组细胞生长状态明显变差,细胞变圆,边缘模糊。结论:ST可以抑制前列腺癌细胞的生长和增殖,诱导细胞凋亡。

Objective： To investigate the effects of staurosporine（ST） on the proliferation and apoptosis of prostate cancer PC-3 cells.Methods： Prostate cancer PC-3 cells were treated in vitro with ST at 10-8mol/L.The expressions of cyclin A and cyclin D1 proteins in the cells were detected by Western blot,the effect of ST on the proliferation of the cells determined by MTT assay and plate colony formation,the apoptosis of the cells examined by flow cytometry,and their morphological changes observed under the light microscope.Results： ST treatment markedly decreased the expressions of cyclin A and cyclin D1 in the PC-3 cells,and significantly inhibited the growth of the PC-3 cells（19.35%） at 48 h.（F = 31.06,P 0.01）.The colony formation rate of the PC-3 cells was（37.10 ± 3.43） % in the ST group,significantly lower than（64.80 ± 4.34） % in the control（χ2 = 14.59,P 0.05） and（62.80 ± 4.36） % in the DMSO group（χ2 =12.50,P 0.05）,while the apoptosis rate of the cells was remarkably higher in the ST group（[19.6 ± 2.20] %） than in the control（[5.33 ± 1.40] %） and the DMSO group（[5.50 ± 0.96] %）（F = 104.36,P 0.01）.Under the light microscope,the ST-treated cells were round with indistinct margins as compared with those of the other two groups.Conclusion： ST could significantly inhibit the proliferation and induce the apoptosis of PC-3 cells.