合并前列腺炎症的良性前列腺增生组织中Integrinα_2β_1/CD133表达及其意义

Expressions of Integrinα_2β_1 and CD133 in benign prostatic hyperplasia complicated by prostatitis and their significance

目的:探讨合并前列腺炎症的良性前列腺增生(BPH)组织中Integrinα2β1/CD133表达及其意义。方法:收集56例行经膀胱前列腺摘除手术的BPH患者的前列腺标本,常规HE染色后观察其合并炎症情况,其中单纯BPH(24例)为A组,合并前列腺炎症的BPH(32例)为B组。采用免疫组化SP法及Western印迹法检测Integrinα2β1/CD133在两组前列腺组织中表达差异。Image-ProPlus 6.0图像分析软件对结果进行分析。结果:免疫组化Mattern积分结果显示:两组中Integrinα2β1/CD133表达有显著差异(P<0.05)。Western免疫印迹显示:B组Integrinα2β1平均相对光密度值高于A组[(0.29±0.18)vs(0.04±0.03)],P<0.05;B组CD133平均相对光密度值高于A组[(0.08±0.07)vs(0.002 0±0.001 8)],P<0.05。提示B组中Integrinα2β1/CD133表达较A组显著升高。结论:炎症可以促进BPH组织中Integrinα2β1/CD133蛋白表达表达。

Objective： To study the expressions of Integrinα2β1 and CD133 in benign prostatic hyperplasia（BPH） complicated by prostatitis and their significance.Methods： Specimens were obtained from 56 BPH patients undergoing transvesical prostatectomy.Paraffin sections of the specimens were subjected to HE staining for pathological examination of inflammatory changes under the light microscope.Twenty-four patients with simple BPH were included in Group A,and the other 32 with BPH complicated with prostatitis in Group B.The expressions of Integrinα2β1 and CD133 in the prostatic tissues of the two groups were determined by immunohistochemistry,Western blotting and IPP6.0 image analysis software.Results： The expressions of Integrinα2β1 and CD133 were significantly higher in Group B than in A（P 0.05）,and so were the mean relative value of the optical density of Integrinα2β1（0.29 ± 0.18 vs 0.04 ± 0.03） and that of CD133（0.08 ± 0.07 vs 0.002 0 ± 0.001 8）（P 0.05）.Conclusion： Inflammation can up-regulate the expressions of Integrinα2β1 and CD133 in BPH tissue.