雷公藤内酯醇对系统性红斑狼疮患者DC细胞功能及成熟的影响

Regulation on function and maturation of dendritic cells from systemic lupus erythematosus patients by triptolide

目的通过休外实验研究雷公藤内酯醇（triptolide）对系统悱红斑狼疮（systemic lupuserythematosus，SI．E）患者树突状细胞（dendriticcell，DC）功能及成熟的影响，为进一步阐明甫公藤内酯醇的免疫学活性提供依据。方法从SLE患者外周血分离单个核细胞，流式细胞仪分选DC，加入0、5、10、30μg／L的雷公藤内酯醇共孵台，24h后收集上清液，ELISA检测IFN-α、TL-6、TNF-α艟，5d后收集细胞，流式细胞仪检测DC表型CDllc、CD80、CD86阳什率，光镜观察DC的肜念，扫描咀镜观察I）C的超微结构。结果雷公藤内酯醇显著减低活动期非活动期SLE患者IFN-α、IL-6、TNF-α量，并呈雷公藤内酯醇浓度依赖性（P〈0．05）；甫公藤内酯醇可抑制SLE心行DC的分化和成熟，外卫雷公藤内酯醇浓度依赖性（P〈0．05）。结论雷公藤内酯醇能够减弱SLE患者DC的功能，并抑制其分化和成熟。

Objective To explore the mechanism of immunomodulatnry activity of triptolide on systemic lupus erythematosus （SLE） patients peripheral blood mononuclear cells （PBMC） -derived dendritic cells （DCs）. Methods SLE -derived DCs were sorted by flow cytometry from peripheral blood mononuclear cells, then incubated with triptolide （0, 5, 10, 30 μg/L ）. After 24 h, we collected the supernatants and then detected the concentration of lFN-α, IL-6, TNF-α using ELISA. After 5 d, the cultrural cells were col- lected and CD11 e, CDS0, CD86 expression of DCs were analyzed by flow cytometry, we ohserved the morphology of DC by light microscope and electron microscope scanning. Results Triptolide -treated DCs from SLE patients with active and stable disease secreted lower level of IFN-α, IL-6, TNF-α triptolide could inhibit DCs differentiation, which displayed more immature morphology and immunophenotypes than untreated- DCs. Conclusion Triptolide could decrease the immune function of DCs from SloE, inhibit differentiation and maturation of DCs.