脑出血大鼠血肿周围脑组织白细胞和凋亡细胞变化及其与脑组织含水量的关系

Change of leukocyte and apoptosis at brain tissue around hematoma and their relationship with brain water content in rats with intracerebral hemorrhage

目的探讨脑出血大鼠血肿周围脑组织白细胞和凋亡细胞变化及其与脑组织含水量的关系。方法 48只大鼠采用自体不凝血注入法制备脑出血模型,随机分为8组:假手术组、脑出血6 h组、脑出血12h组、脑出血24 h组、脑出血48 h组、脑出血72 h组、脑出血1周组和脑出血2周组。分别在相应时间点断头取脑,进行脑组织含水量测定和HE染色、TUNEL染色,计数各组大鼠血肿周围脑组织白细胞数及凋亡细胞数。结果脑出血后6 h^1周内大鼠脑组织含水量均明显高于假手术组,其中脑出血72 h时大鼠脑组织含水量最高(均P<0.05)。假手术组及脑出血6 h组、脑出血2周组均无白细胞表达;脑出血后12 h^1周内大鼠血肿周围脑组织均可见散在的白细胞浸润,其中脑出血48 h时白细胞数最多(均P<0.05)。各时间点脑出血大鼠凋亡细胞数均明显高于假手术组,其中脑出血72 h时凋亡细胞数最高(均P<0.05)。脑出血后血肿周围脑组织白细胞数和凋亡细胞数分别与脑组织含水量呈正相关(r=0.615,P<0.05;r=0.578,P<0.01)。结论脑出血大鼠血肿周围脑组织白细胞和凋亡细胞增加可能与脑水肿的形成有关。

Objective To explore the change of leukocyte and apoptosis at brain tissue around hematoma and their relationship with brain water content in rats with intracerebral hemorrhage（ICH）.Methods ICH models was made by nonthrombogenic autologous blood injection method in 48 rats,and then the rats were randomly divided into 8 groups： sham operation group,ICH 6 h group,ICH 12 h group,ICH 24 h group,ICH 48 h group,ICH 72 h group,ICH 1 week group and ICH 2 weeks group.The brain of rats was taken out at corresponding time point respectively,and the brain water content was measured.The HE staining and TUNEL staining was made and the leukocyte and apoptosis were counted.Results The brain water content of rats at 6 h-1 week after ICH was significantly higher than sham operation group,and which was the highest at 72 h after ICH（all P0.05）.The leukocyte was not found at sham operation group,ICH 6 h group and ICH 2 weeks group.The leukocyte was interspersed and infiltrated at 12 h-1 week after ICH,and which was the most at 48 h after ICH（all P0.05）.The number of apoptosis of ICH rats at each time point was significantly higher than sham operation group,and which was the highest at 72 h after ICH（all P0.05）.The leukocyte and apoptosis at brain tissue around hematoma were both positively related with the brain water content（r=0.615,P0.01;r=0.578,P0.01）.Conclusion The increase of leukocyte and apotosis at brain tissue around hematoma in ICH rats may be related with the formation of encephaledema.