睡眠剥夺疲劳模型大鼠脑电分析

EEG Analysis of Rats Fatigue Model during Sleep Deprivation

目的通过睡眠剥夺(SD)疲劳模型大鼠造模过程中动态脑电的分析,研究疲劳程度随睡眠剥夺时间的动态变化。方法本文采用小水站台睡眠剥夺动物疲劳模型,连续72 h记录造模过程中以及恢复48 h大鼠的行为和脑电信号;应用自行开发的脑电分析程序计算分析时间窗内脑电的α,θ,β波频谱能量占总能量的百分比及(α+θ)β/比值;并分析随睡眠剥夺时间的延长,清醒活动(WA)、清醒安静(WQ)及慢波睡眠(SWS)状态下各参数的变化。结果在睡眠剥夺后,β波频带能量占总能量百分比参数在3种状态下均呈上升趋势,(α+θ)β/比值呈下降趋势;2个参数在清醒活动状态下持续变化到35h左右达到峰值并维持,恢复24 h后参数可回到基线水平;清醒安静状态的变化延迟约20 h,但同样在40 h左右达到峰值并维持,恢复期参数值回复缓慢;慢波睡眠状态下参数睡眠剥夺初期变化剧烈,但10h后即持续缓慢回复,70 h左右达基线水平。结论小站台睡眠剥夺疲劳模型实验对大鼠脑电特征有明显影响,β波频谱能量占总能量的百分比和(α+θ)β/值可作为中枢疲劳程度的评价指标。

Objective To explore the fatigue level changes of rats during the sleep deprivation（SD）.Methods The SD model was induced with flower-pot technique.The rat＇s EEG and behavior were recorded continuously during over 72 h SD and 48 h recovery.With EEG analysis software developed by ourselves,the percentage of different frequency components to overall power spectrum（α%,θ%,β%）,and the ratio of α＋θ to β were calculated,the variance of these parameters in waking quiet state（WQ）,waking active state（WA） and slow wave sleep state（SWS） were analyzed.Results At the beginning of SD,the percentage of β increased in all 3 states,but the ratio of α＋θ to β decreased at WQ delayed about 15 h.At WQ and WA,the increasing or decreasing lasted for 40 h,followed by a relatively steady period.At SWS,the increasing of β% and the decreasing of（α＋θ）/β only lasted for about 10 h,and then the direction of variance were changed.The 2 parameters could be recovered in WA after 48 h recovery,but not in WQ and SWS.Conclusion The percentage of β band and the value of（α＋θ）/β can be used as an index of central fatigue.