荧光定量PCR检测肝胆癌组织内HCV RNA含量

HCV RNA Quantification in the Tissues of Hepatobiliary Tumors by Fluorescent Quantitative PCR

目的:检测肝胆癌组织内丙型肝炎病毒(HCV)RNA表达。方法:收集手术切除的新鲜肝胆肿瘤组织标本共31例,其中胆道肿瘤14例,肝癌11例,肝硬化6例,采用荧光定量PCR方法检测HCV RNA含量;同时采用酶联免疫吸附法检测患者血清抗-HCV。结果:胆道肿瘤中无1例HCV RNA阳性,1例肝癌组织和1例肝硬化组织可检测到HCV RNA含量,且其相应的血清抗-HCV阳性。结论:胆道肿瘤组织内HCV RNA阳性率极低,目前尚难定论HCV就是胆管癌发生的危险因素之一。

Objective To assay HCV RNA content in the samples from patients with hepatobiliary tumors.Methods Total 31 fresh surgically ablated samples were obtained from 14 cases with biliary tract carcinomas,11 cases with liver cancers and 6 cases with liver cirrhosis.HCV RNA in the tissues was quantified by fluorescent quantitative polymerase chain reaction （FQ-PCR）.Enzyme-linked immunosorbent assay was applied to detect serum anti-HCV.Results None of samples from biliary tract carcinomas had detected HCV RNA.HCV RNA quantification was assayed only in one liver cancer sample and one liver cirrhosis tissue.Correspondingly,serum anti-HCV was also positive in the two cases.Conclusion Few tissues from biliary tract carcinomas can be detected HCV RNA.It is inconclusive whether HCV is one of the risk factors for carcinogenesis of biliary tract carcinoma.