摘要
目的:研究高血压病患者大网膜小动脉病理损害、重塑及相关蛋白的表达。方法:检测12例非高血压病对照者和12例高血压病患者血液流变学及大网膜小动脉形态学的变化,采用免疫组织化学方法检测大网膜小动脉Bcl-2、Bax、c-Myc、c-Fos、MMP-9和TIMP-2蛋白表达,用Masson染色法观察大网膜小动脉壁胶原含量变化。结果:高血压病患者血液流变学异常,大网膜小动脉胶原沉积增多,中膜厚度/管腔直径比值增大,动脉壁Bcl-2、Bax、c-Myc、c-Fos、MMP-9和TIMP-2蛋白表达增强。结论:高血压病患者大网膜小动脉存在病理损害和重塑,其机制与血液动力学(高血压)和血液流变学异常,动脉壁Bcl-2和Bax、MMP-9和TIMP-2表达失衡及c-Myc和c-Fos过度表达有关。
AIM:To study the pathological changes,adverse remodeling and related protein expression of omental arteriole in hypertensive patients.METHODS: Twelve non-hypertensive persons and 12 hypertensive patients were selected,and the hemorheology and morphological changes of their omental arterioles were detected.The protein expression of Bcl-2,Bax,c-Myc,c-Fos,MMP-9 and TIMP-2 in the arterioles of omental tissues was determined by the method of immunohistochemistry.The changes of collagen content in omental arterioles were observed by Masson staining.RESULTS: Compared with the non-hypertensive persons,the hemorheological abnormalities,increased omental arteriole collagen deposition,widened mid layer thickness/lumenal diameter ratio and enhanced protein expression of Bcl-2,Bax,c-Myc,c-Fos,MMP-9 and TIMP-2 were observed in hypertensive patients.CONCLUSION: The pathological damage and adverse remodeling exist in omental arterioles of hypertensive patients.The mechanism may be related with the hymodynamic of hypertension,hemorheological abnormalities,imbalanced expression of Bcl-2,Bax,MMP-9 and TIMP-2,and concerned with the over-expression of c-Myc and c-Fos in the arteriolar wall.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第10期1863-1867,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30300466)
泰山学者建设工程专项经费资助项目(No.鲁办发[2004]28号)