高血压病患者大网膜小动脉重塑的观测及相关蛋白表达探讨

Omental arteriole remodeling and related protein expression in hypertensive patients

目的:研究高血压病患者大网膜小动脉病理损害、重塑及相关蛋白的表达。方法:检测12例非高血压病对照者和12例高血压病患者血液流变学及大网膜小动脉形态学的变化,采用免疫组织化学方法检测大网膜小动脉Bcl-2、Bax、c-Myc、c-Fos、MMP-9和TIMP-2蛋白表达,用Masson染色法观察大网膜小动脉壁胶原含量变化。结果:高血压病患者血液流变学异常,大网膜小动脉胶原沉积增多,中膜厚度/管腔直径比值增大,动脉壁Bcl-2、Bax、c-Myc、c-Fos、MMP-9和TIMP-2蛋白表达增强。结论:高血压病患者大网膜小动脉存在病理损害和重塑,其机制与血液动力学(高血压)和血液流变学异常,动脉壁Bcl-2和Bax、MMP-9和TIMP-2表达失衡及c-Myc和c-Fos过度表达有关。

AIM：To study the pathological changes,adverse remodeling and related protein expression of omental arteriole in hypertensive patients.METHODS： Twelve non-hypertensive persons and 12 hypertensive patients were selected,and the hemorheology and morphological changes of their omental arterioles were detected.The protein expression of Bcl-2,Bax,c-Myc,c-Fos,MMP-9 and TIMP-2 in the arterioles of omental tissues was determined by the method of immunohistochemistry.The changes of collagen content in omental arterioles were observed by Masson staining.RESULTS： Compared with the non-hypertensive persons,the hemorheological abnormalities,increased omental arteriole collagen deposition,widened mid layer thickness/lumenal diameter ratio and enhanced protein expression of Bcl-2,Bax,c-Myc,c-Fos,MMP-9 and TIMP-2 were observed in hypertensive patients.CONCLUSION： The pathological damage and adverse remodeling exist in omental arterioles of hypertensive patients.The mechanism may be related with the hymodynamic of hypertension,hemorheological abnormalities,imbalanced expression of Bcl-2,Bax,MMP-9 and TIMP-2,and concerned with the over-expression of c-Myc and c-Fos in the arteriolar wall.