摘要
目的通过Real Time PCR实时监测慢性粒细胞白血病bcr/abl融合基因的表达,探讨其在诊断及预后评价价值。方法采用荧光定量Real Time PCR反应技术检测39例慢性粒细胞白血病及其它血液系统疾病20例的bcr/abl融合基因表达水平。结果骨髓原始、外周血原始与bcr/abl融合基因拷贝数有显著相关性,初诊慢性粒细胞的bcr/abl融合基因表达水平普遍明显增高,经羟基脲±干扰素治疗后表达明显下降,复发或加速期患者可提前2-4个月左右早期发现,格列卫或移植治疗后,多数bcr/abl融合基因可转阴性。结论 bcr/abl融合基因检测对诊断慢性粒细胞白血病有重要价值,同样是慢性粒细胞白血病治疗和预后观察的决定性指标。
Objective To investigate the value of diagnosis and prognostic evaluation according to detect the expression of bcr/abl fusion gene in chronic myeloid leukemia by real-time quatitative PCR.Methods To use the real-time PCR to detect the expression of bcr/abl fusion gene of 39 cases chronic myeloid leukemia and 20 cases others hematological diseases.Results Marrow and peripheral blasts showed significant corrilation with the copy of bcr/abl fusion gene.Bcr/abl fusion gene expression level of newly diagnosis chronic myeloid leukemia increased significantly.After the treatment of hydroxycarbamide±interferon,the expression level of the gene decreased markedly.The patients of recurrence or acceleration phase can be found ahead of 2-4 months By the therapy of Glevec or explantation,the most of the fusion gene turned negative.Conclusion The detection of bcr/abl fusion gene is an important way to diagnose chronic myeloid leukemia,and can be a decisive index of treatment and prognostic evaluation.
出处
《中国实验诊断学》
北大核心
2011年第10期1657-1659,共3页
Chinese Journal of Laboratory Diagnosis
基金
江西省卫生厅2006年普通计划课题(课题号20063160)
