亚低温状态5周游泳运动联合干预对NAFLD大鼠肝组织HSP70mRNA的影响

Influence of 5-week Swimming Exercise on the HSP70 mRNA of Liver Tissue of SD Rat Model of Dietary-induced NAFLD under Mild Hypothermia

目的:探讨在非酒精性脂肪肝病(NAFLD)动物模型建造过程中实施亚低温状态5周游泳运动联合干预对诱导NAFLD大鼠肝组织HSP70mRNA表达量及其HSP70生成量变化的影响,旨在研究延缓NAFLD的机制。方法:60只SD大鼠随机分为对照组(C)、高脂组(F)、常温游泳高脂组(TS)、常温浸泡高脂组(TI)、亚低温游泳高脂组(HS)和亚低温浸泡高脂组(HI)各10只。建造NAFLD动物模型的过程中,除C组外均给予相应高脂液体饲料喂养,并分别进行不同温度的游泳或浸泡干预。5周后采用固相夹心法酶联免疫吸附实验(ELISA)检测各组肝脏组织HSP70浓度和Real-Time PCR相对定量测试HSP70mRNA。结果:肝组织HSP70浓度及HSP70mRNA,HS组均明显高于C、F组(P<0.01)。结论:在SD大鼠NAFLD模型建造过程中,实施亚低温状态下联合有氧运动干预措施,可以诱导NAFLD肝细胞生成大量应激蛋白,而延缓NAFLD的发生与发展。

Objective： To explore the mechanism of delaying dietary-induced NAFLD by studying the influence of 5-week swimming exercise on the HSP79 mRNA of liver tissue of SD rat model of dietary-induced NAFLD under the mild hypothermia.Methods： Sixty SD rats were randomly divided into control group（C）,high-fat feeding group（F）,group of high-fat feeding plus swimming at room temperature（TS）,room temperature immersion plus high-fat feeding group（TI）,mild hypothermia swimming plus high fat feeding group（HS）,and the hypothermia immersion plus high-fat feeding group（HI）.Control group was given normal feeding solids and other high-fat feeding groups were given the liquid preparation and high-fat feeding for 5 weeks.SD rats were killed after 5 weeks.The expression of HSP70 protein and mRNA in the liver tissue was detected by using ELISA and real-time PCR respectively.Results： HSP70 expression in liver tissue was significantly increased in HS group as compared with groups C and F.The HSP70 mRNA in the liver tissue in HS group was significantly increased as compared with groups C and F.Conclusion： In the process of SD rat NAFLD model establishment,the intervention of mild hypothermia in combination with swimming exercise could induce the production of HSP in the liver tissue of SD rat NAFLD model,and delay the development of NAFLD.