应用RAPD和ISSR标记对24份花生栽培种材料进行遗传多样性分析

Genetic diversity in 24 peanut cultivars as revealed by RAPD/ISSR profiling

利用RAPD引物和ISSR引物分析我国24份花生栽培种材料的遗传多样性。结果表明:所选的RAPD引物和ISSR引物中分别有13条引物和10条引物扩增出了清晰并可重复的条带,共扩增出123条带和87条带,平均每条引物扩增出9.5条带和8.7条带,其中多态性带分别占条带总数的47.15%和57.47%,平均每条引物扩增出4.7条和5.7条多态性带。在此基础上,根据Nei-Li系数采用UPGMA法进行了聚类分析,可将24份花生材料分成4类:四粒红、汕油523、冀花2号、花育16和粤油7号等5个品种聚为一类;花育20、中花8号聚为一类;黑花生单独为一类;其余的花生聚为一类。RAPD和ISSR标记能够揭示花生栽培种的遗传多样性,在种质鉴定和遗传作图等方面具有一定应用潜力。

Genetic diversity in 24 accessions of cultivated peanut materials from China was evaluated based on RAPD/ ISSR profiling. Of the RAPD primers and ISSR primers tested, 13 and 10 primers produced a total of 123 and 87 repeatable,clear and readable bands,among which 47. 15% and 57. 47% were polymorphic,respectively. On average,a primer resulted in 9.5 and 8. 7 bands, of which 4. 7 and 5.7 were polymorphic. A dendrogram was constructed using UPGMA algorithm based on Nei and Li＇s similarity coefficient, which divided the 24 peanut materials into 4 groups. Silihong,Sanyou 523 ,Jihua 2, Huayu 16 and Yueyou 7 were in one group. Huyu 20 and Zhonghua 8 clustered together. Heihuasheng formed a separated group, and the rest peanut materials tested fell in a group. RAPD and ISSR are useful for the genetic diversity studies of the cultivated peanut,and have potential in characterization of peanut germ plasm and gene mapping.