连香树生理生化物质随海拔的变化及其适应性研究

Research on physiological and biochemical material’s change of Cercidiphyllum japonicum at different altitudes and adaptability

通过分析四川甘洛马鞍山自然保护区内不同海拔(A1:1874m,A2:1996m,A3:2160m,A4:2290m)梯度连香树的分布状况以及生理指标含量的变化,探讨了连香树对不同环境的适应能力。结果表明:随着海拔的升高,样方内连香树的总数、株高、幼树数量及树冠投影面积值均呈降低趋势,且海拔越高,其它物种抢占的空间资源越多以及环境变恶劣,连香树的适应能力下降;各种生理指标的变化为:叶绿素a、叶绿素b、叶绿素(a+b)和类胡萝卜素含量在A1处均高于A2、A3、A4处。A4与A1相比,叶绿素b、叶绿素(a+b)分别下降了42.42%和33.96%,差异显著。超氧化物歧化酶(SOD)活性、可溶性糖含量均随着海拔升高呈下降趋势,A4与A1相比,分别下降了52.74%、66.17%,差异显著;丙二醛(MDA)和游离脯氨酸(Pro)含量均随着海拔升高呈上升趋势,A4与A1相比,分别升高了3.98倍、4.43倍,差异显著;可溶性蛋白含量呈先降后升趋势,A1、A3、A4处均高于A2处。综合本次研究表明:A1处最适合连香树的生长、A4处连香树生长最差。

To study the environment adaptability of Cercidiphyllum japonicum in Maanshan Nature Reserve in Ganluo County of Sichuan Province, the distribution conditions and contents of physiological indexes of C. japonicumat different altitudes（A1 ： 1 874 m; A2 ： 1 996 m; A3 ： 2 160 m; A4 ： 2 290 m） were investigated. The results showed that the total number and the height of trees, the number of young trees and the range of tree crown decreased with increasing altitude. The higher the altitude was, the more spatial resources occupied by other species were. So the environment adaptability of C. japonicum reduced. The change of physiological indexes showed that：contents of Chla, Chlb,Chl（a＋b） and Car at A1 were higher than those at A2 , A3, A4. Compared with A1, contents of Chlb and Chl （a＋b）decreased significantly 42. 42 %, 33.96 % at A4. Soluble sugar and SOD activities decreased with increasingaltitude. Compared with those at Al,they decreased significantly 52. 74%, 66.17 % at A4; MDA and Pro contents increased with increasing altitude. At A4 they increased significantly 3.98 time and 4.43 time compared with those in A1. The soluble protein content first decreased then increased. Protein content at A1, A3, A4 were higher than that in A4. In general, it is conducive to the growth and development of C. japonicum at A1 and they grow badly at A4.